Optimization of enzymatic saccharification of industrial wastes using a thermostable and halotolerant endoglucanase through Box-Behnken experimental design

Abstract This study describes the production, characterization and application of an endoglucanase from Penicillium roqueforti using lignocellulosic agro-industrial wastes as the substrate during solid-state fermentation. The endoglucanase was generated after culturing with different agro-industrial wastes for 96 h without any pretreatment. The highest activity was obtained at 50 °C and pH 4.0. Additionally, the enzyme showed stability in the temperature and pH ranges of 40–80 °C and 4.0–5.0, respectively. The addition of Ca2+, Zn2+, Mg2+, and Cu2+ increased enzymatic activity. Halotolerance as a characteristic of the enzyme was confirmed when its activity increased by 35% on addition of 2 M NaCl. The endoglucanase saccharified sugarcane bagasse, coconut shell, wheat bran, cocoa fruit shell, and cocoa seed husk. The Box-Behnken design was employed to optimize fermentable sugar production by evaluating the following parameters: time, substrate, and enzyme concentration. Under ideal conditions, 253.19 mg/g of fermentable sugars were obtained following the saccharification of wheat bran, which is 41.5 times higher than that obtained without optimizing. This study presents a thermostable, halotolerant endoglucanase that is resistant to metal ions and organic solvents with the potential to be applied in producing fermentable sugars for manufacturing biofuels from agro-industrial wastes. GRAPHICAL ABSTRACT HIGHLIGHTS Penicillum roqueforti ATCC 10110 produced an endoglucanase in culture media containing only agro-industrial wastes. The exploitation of agro-industrial wastes increases the added economic value of these materials and facilitates the management of solid wastes. SEM confirmed the structural changes in the waste biomass induced by saccharification. The Box-Behnken design, used for saccharification optimization, increased the production of fermentable sugars by 41.5 times without any pretreatment of the agro-industrial wastes.

• Publication year

  2023

• Venue

  Preparative Biochemistry & Biotechnology

• Publication date

  2023-04-18

• Fields of study

  Medicine, Chemistry, Engineering, Environmental Science

• Identifiers
  DOI 10.1080/10826068.2023.2201936PMID 37071540
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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