A functional eukaryotic promoter is contained within the first intron of the hGH-N coding region.

Human growth hormone is frequently used as a reporter gene in studies addressing the regulation of eukaryotic promoters. Here we present evidence that the first intron of the hGH-N coding region contains a novel eukaryotic promoter which is able to direct the expression of hGH-N and luciferase reporter genes. The corresponding transcriptional initiation site is located downstream of the splice acceptor site of exon 2. This internal hGH-N promoter can interfere with the transcriptional control elements of a promoter linked to the hGH-N coding region and thereby complicate the interpretation of data obtained with hGH-N as a reporter gene.

• Publication year

  1998

• Venue

  Biochemical and Biophysical Research Communications - BBRC

• Publication date

  1998-06-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1006/BBRC.1998.8782PMID 9642126
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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