Exploring bacterial cell-free supernatants, unfiltered ferments and crude bacteria uses of Xenorhabdus and Photorhabdus (Morganellaceae) for controlling Botrytis cinerea (Helotiales: Sclerotiniaceae)

well-known associated biological control agents that produce a diverse range of natural antifungal compounds. This study aimed to evaluate the efficacy of different control strategies against B. cinerea using: ( i ) EPN symbiotic bacterial cell-free supernatants, ( ii ) unfiltered ferments and ( iii ) crude bacteria isolates. The antifungal efficacy of X. bovienii , X. nematophila, X. kozodoii and P. laumondii subsp. lau-mondii cell-free supernatants obtained after the bacterial fermentations were tested in vitro at two different concentrations (10% and 20%). Furthermore, the antifungal effect of X. nematophila and P. laumondii unfiltered ferments were tested in vitro , and their dissuasive effect was also tested over tomato leaves. Finally, the anti-fungal capacity of the crude X. nematophila and P. laumondii isolate was tested comparing their effect with the fungicide effect of the commercial Bacillus amyloliquefaciens (Serenade ® ASO fungicide). The results showed that employing the bacterial cell-free supernatants or the unfiltered ferments could have different antifungal efficacy against this pathogen. Applying X. nematophila cell-free supernatant and unfiltered ferments at 20% concentration resulted in the highest inhibition effect compared with the control (distilled water), 82% and 100%, respectively. Furthermore, P. laumondii -isolate can control the growth of Botrytis in in vitro conditions, showing

• Publication year

  2023

• Venue

  Biological control (Print)

• Publication date

  2023-08-01

• Fields of study

  Not labeled

• Identifiers
  DOI 10.1016/j.biocontrol.2023.105259
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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