Impact of a wastewater treatment plant on microbial community composition and function in a hyporheic zone of a eutrophic river

The impact of the installation of a technologically advanced wastewater treatment plant (WWTP) on the benthic microbial community of a vinyl chloride (VC) impacted eutrophic river was examined two years before and three and four years after installation of the WWTP. Reduced dissolved organic carbon and increased dissolved oxygen concentrations in surface water and reduced total organic carbon and total nitrogen content in the sediment were recorded in the post-WWTP samples. Pyrosequencing of bacterial 16S rRNA gene fragments in sediment cores showed reduced relative abundance of heterotrophs and fermenters such as Chloroflexi and Firmicutes in more oxic and nutrient poor post-WWTP sediments. Similarly, quantitative PCR analysis showed 1–3 orders of magnitude reduction in phylogenetic and functional genes of sulphate reducers, denitrifiers, ammonium oxidizers, methanogens and VC-respiring Dehalococcoides mccartyi. In contrast, members of Proteobacteria adapted to nutrient-poor conditions were enriched in post-WWTP samples. This transition in the trophic state of the hyporheic sediments reduced but did not abolish the VC respiration potential in the post-WWTP sediments as an important hyporheic sediment function. Our results highlight effective nutrient load reduction and parallel microbial ecological state restoration of a human-stressed urban river as a result of installation of a WWTP.

• Publication year

  2015

• Venue

  Scientific Reports

• Publication date

  2015-11-26

• Fields of study

  Medicine, Environmental Science

• Identifiers
  DOI 10.1038/srep17284PMID 26607034PMCID 4660315
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Use of pyrosequencing to explore the benthic bacterial community structure in a river impacted by wastewater treatment plant discharges.

  2014cited by this paper
• Characterization of Bacterial Communities in Sediments Receiving Various Wastewater Effluents with High-Throughput Sequencing Analysis

  2014cited by this paper
• R: A language and environment for statistical computing.

  2014cited by this paper
• phyloseq: An R Package for Reproducible Interactive Analysis and Graphics of Microbiome Census Data

  2013cited by this paper
• Small-scale oxygen distribution determines the vinyl chloride biodegradation pathway in surficial sediments of riverbed hyporheic zones.

  2013cited by this paper
• Community genomic analyses constrain the distribution of metabolic traits across the Chloroflexi phylum and indicate roles in sediment carbon cycling

  2013cited by this paper
• Waste Not, Want Not: Why Rarefying Microbiome Data Is Inadmissible

  2013cited by this paper
• Evaluation of solid polymeric organic materials for use in bioreactive sediment capping to stimulate the degradation of chlorinated aliphatic hydrocarbons

  2013cited by this paper
• The xylan utilization system of the plant pathogen Xanthomonas campestris pv campestris controls epiphytic life and reveals common features with oligotrophic bacteria and animal gut symbionts.

  2013cited by this paper
• Wastewater Treatment Effluent Reduces the Abundance and Diversity of Benthic Bacterial Communities in Urban and Suburban Rivers

  2013cited by this paper
• Release and Consumption of d-Amino Acids During Growth of Marine Prokaryotes

  2013cited by this paper
• Metagenomic analysis of a stable trichloroethene-degrading microbial community

  2012cited by this paper
• Comparative metagenomics of three Dehalococcoides-containing enrichment cultures: the role of the non-dechlorinating community

  2012cited by this paper
• Fast, accurate error-correction of amplicon pyrosequences using Acacia

  2012cited by this paper
• Temporal variations in natural attenuation of chlorinated aliphatic hydrocarbons in eutrophic river sediments impacted by a contaminated groundwater plume.

  2012cited by this paper
• Common Features of Environmental and Potentially Beneficial Plant-Associated Burkholderia

  2012cited by this paper
• Dysfunction of the intestinal microbiome in inflammatory bowel disease and treatment

  2012cited by this paper
• Pyrosequencing Reveals the Influence of Organic and Conventional Farming Systems on Bacterial Communities

  2012cited by this paper
• Diversity, abundance, and activity of ammonia-oxidizing bacteria and archaea in Chongming eastern intertidal sediments

  2012cited by this paper
• Bacterial Communities in the Sediments of Dianchi Lake, a Partitioned Eutrophic Waterbody in China

  2012cited by this paper
• Microarray Analysis and Barcoded Pyrosequencing Provide Consistent Microbial Profiles Depending on the Source of Human Intestinal Samples

  2011cited by this paper
• Bacterial community dynamics in the hyporheic zone of an intermittent stream

  2011cited by this paper
• UCHIME improves sensitivity and speed of chimera detection

  2011cited by this paper
• QIIME allows analysis of high-throughput community sequencing data

  2010cited by this paper
• A review of allochthonous organic matter dynamics and metabolism in streams

  2010cited by this paper
• Characterizing seasonal changes in physicochemistry and bacterial community composition in hyporheic sediments

  2010cited by this paper
• Cultivation of uncultured chloroflexi subphyla: significance and ecophysiology of formerly uncultured chloroflexi 'subphylum i' with natural and biotechnological relevance.

  2009cited by this paper
• Contribution of Wastewater Treatment Plant Effluents to Nutrient Dynamics in Aquatic Systems: A Review

  2009cited by this paper
• Factors determining the attenuation of chlorinated aliphatic hydrocarbons in eutrohic river sediment impacted by discharging polluted groundwater.

  2009cited by this paper
• Evaluating the fate of chlorinated ethenes in streambed sediments by combining stable isotope, geochemical and microbial methods.

  2009cited by this paper
• The Relation of Genetic and Environmental Factors to Systemic Inflammatory Biomarker Concentrations

  2009cited by this paper
• Assessment of the intrinsic bioremediation capacity of an eutrophic river sediment polluted by discharging chlorinated aliphatic hydrocarbons: a compound-specific isotope approach.

  2009cited by this paper
• Habitat Heterogeneity and Associated Microbial Community Structure in a Small-Scale Floodplain Hyporheic Flow Path

  2009cited by this paper
• Effect of Wastewater Treatment Plant Effluent on Microbial Function and Community Structure in the Sediment of a Freshwater Stream with Variable Seasonal Flow

  2008cited by this paper
• Differentiated free-living and sediment-attached bacterial community structure inside and outside denitrification hotspots in the river–groundwater interface

  2008cited by this paper
• Global Change and the Ecology of Cities

  2008cited by this paper
• The ecology and biotechnology of sulphate-reducing bacteria

  2008cited by this paper
• SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB

  2007cited by this paper
• Methane and sulfate profiles within the subsurface of a tidal flat are reflected by the distribution of sulfate-reducing bacteria and methanogenic archaea.

  2007cited by this paper
• Assessing the impact of VOC-contaminated groundwater on surface water at the city scale.

  2007cited by this paper
• Distribution of the Mycobacterium community and polycyclic aromatic hydrocarbons (PAHs) among different size fractions of a long-term PAH-contaminated soil.

  2006cited by this paper
• Analysis of Diversity and Activity of Sulfate-Reducing Bacterial Communities in Sulfidogenic Bioreactors Using 16S rRNA and dsrB Genes as Molecular Markers

  2006cited by this paper
• Effects of wastewater treatment plant discharge on ecosystem structure and function of lowland streams

  2006cited by this paper
• Recurring Seasonal Dynamics of Microbial Communities in Stream Habitats

  2006cited by this paper
• Quantitative Detection of the nosZ Gene, Encoding Nitrous Oxide Reductase, and Comparison of the Abundances of 16S rRNA, narG, nirK, and nosZ Genes in Soils

  2006cited by this paper
• Biological Filtration Limits Carbon Availability and Affects Downstream Biofilm Formation and Community Structure

  2006cited by this paper
• Phylogeography of Sulfate-Reducing Bacteria among Disturbed Sediments, Disclosed by Analysis of the Dissimilatory Sulfite Reductase Genes (dsrAB)

  2005cited by this paper
• Exploration of riffle-scale interactions between abiotic variables and microbial assemblages in the hyporheic zone

  2005cited by this paper
• Dynamics of an Oligotrophic Bacterial Aquifer Community during Contact with a Groundwater Plume Contaminated with Benzene, Toluene, Ethylbenzene, and Xylenes: an In Situ Mesocosm Study

  2005cited by this paper
• Development of a real-time PCR method for quantification of the three genera Dehalobacter, Dehalococcoides, and Desulfitobacterium in microbial communities.

  2004cited by this paper
• Characterization of a Highly Enriched Dehalococcoides-Containing Culture That Grows on Vinyl Chloride and Trichloroethene

  2004cited by this paper
• Seasonal Dynamics of Shallow-Hyporheic-Zone Microbial Community Structure along a Heavy-Metal Contamination Gradient

  2004cited by this paper
• Quantification of denitrifying bacteria in soils by nirK gene targeted real-time PCR.

  2004cited by this paper
• Insights into the genomic basis of niche specificity of Pseudomonas putida KT2440.

  2004cited by this paper
• Reassessing PCR primers targeting nirS, nirK and nosZ genes for community surveys of denitrifying bacteria with DGGE.

  2004cited by this paper
• Metabolic and structural response of hyporheic microbial communities to variations in supply of dissolved organic matter

  2003cited by this paper
• Imperilled Subsurface Waters in Australia: Biodiversity, Threatening Processes and Conservation

  2003cited by this paper
• Structure and seasonal dynamics of hyporheic zone microbial communities in free-stone rivers of the estern United States

  2003cited by this paper
• THE CONTROL OF THE FALSE DISCOVERY RATE IN MULTIPLE TESTING UNDER DEPENDENCY

  2001cited by this paper
• The hyporheic zone: Linking groundwater and surface water—understanding the paradigm

  2001cited by this paper
• Comparison of Methods for Quantification of Cytochrome cd1-Denitrifying Bacteria in Environmental Marine Samples

  2000cited by this paper
• The domain-specific probe EUB338 is insufficient for the detection of all Bacteria: development and evaluation of a more comprehensive probe set.

  1999cited by this paper
• Metabolism of streamwater dissolved organic carbon in the shallow hyporheic zone

  1993cited by this paper
• Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA

  1993cited by this paper
• 16S/23S rRNA sequencing

  1991cited by this paper
• Nucleic acid techniques in bacterial systematics

  1991cited by this paper
• The Statistical Analysis of Compositional Data

  1986cited by this paper

• A 10-year investigation of the impact of a wastewater treatment plant upgrade on nitrogen-cycling microorganisms and DNA viruses in receiving river

  2026cites this paper
• How does fertilization impact the wild blueberry microbiome?

  2025cites this paper
• Metagenomic Analysis of Biofloc Produced from Furfural Residue Wastewater Treatment

  2025cites this paper
• Wastewater-effluent discharge and incomplete denitrification drive riverine CO2, CH4 and N2O emissions.

  2024cites this paper
• High-proportions of tailwater discharge alter microbial community composition and assembly in receiving sediments

  2024cites this paper
• Lotic Ecosystem Sediment Microbial Communities’ Resilience to the Impact of Wastewater Effluents in a Polluted European Hotspot—Mureș Basin (Transylvania, Romania)

  2024cites this paper
• Response of hyporheic biofilms to temperature changes and dissolved organic carbon enrichment: a mesocosm study

  2024cites this paper
• Metagenomics insights into bacterial diversity and antibiotic resistome of the sewage in the city of Belém, Pará, Brazil

  2024cites this paper
• Variability of Biodegradation Rates of Commercial Chemicals in Rivers in Different Regions of Europe

  2024cites this paper
• Spatial and temporal metagenomics of river compartments reveals viral community dynamics in an urban impacted stream

  2023cites this paper
• River Sediments Downstream of Villages in a Karstic Watershed Exhibited Increased Numbers and Higher Diversity of Nontuberculous Mycobacteria

  2023cites this paper
• Impact of wastewater treatment upgrade and nitrogen removal on bacterial communities and their interactions in eutrophic prairie streams

  2023influential citation
• Phylogenetic and ecophysiological novelty of subsurface mercury methylators in mangrove sediments

  2023cites this paper
• Spatial and temporal distribution of the microbial community structure in the receiving rivers of the middle and lower reaches of the Yangtze River under the influence of different wastewater types.

  2023cites this paper
• Wastewater discharges alter microbial community composition in surface waters of the canadian prairies.

  2023cites this paper
• Wastewater treatment plant effluent discharge decreases bacterial community diversity and network complexity in urbanized coastal sediment.

  2023cites this paper
• Geography, taxonomy, and ecological guild: Factors impacting freshwater macroinvertebrate gut microbiomes

  2022cites this paper
• Differences in Carbon and Nitrogen Migration and Transformation Driven by Cyanobacteria and Macrophyte Activities in Taihu Lake

  2022cites this paper
• Sediment sulfate content determines assembly processes and network stability of bacteria communities of coastal land-based shrimp aquaculture ponds

  2022cites this paper
• Endospores associated with deep seabed geofluid features in the eastern Gulf of Mexico

  2022cites this paper
• Multi-stage hydrothermal liquefaction modeling of sludge and microalgae biomass to increase bio-oil yield

  2022cites this paper
• The microbial diversity in industrial effluents makes high-throughput sequencing-based source tracking of the effluents possible.

  2022cites this paper
• Insights into the microbial community of treated wastewater, its year-round variability and impact on the receiver, using cultivation, microscopy and amplicon-based methods.

  2022cites this paper
• Microbial diversity analysis of Hudiara Drain wastewater using culture-independent and culture-dependent approaches

  2022cites this paper
• River Biofilms Microbiome and Resistome Responses to Wastewater Treatment Plant Effluents Containing Antibiotics

  2022cites this paper
• Bacterial Indicators Are Ubiquitous Members of Pelagic Microbiome in Anthropogenically Impacted Coastal Ecosystem

  2022cites this paper
• Potential Role of Beneficial Microbes for Sustainable Treatment of Sewage Sludge and Wastewater

  2022cites this paper
• Irrigation suitability of White River in Indiana, Midwestern USA

  2021influential citation
• Deciphering the black box of microbial community of common effluent treatment plant through integrated metagenomics: Tackling industrial effluent.

  2021cites this paper
• Historical exposure to wastewater disposal reinforces the stability of sediment bacterial community in response to future disturbance

  2021cites this paper
• A Mathematical Model Development for Simulating Nitrate Pollutant Transport along a River

  2021cites this paper
• Riparian Land Use and Hydrological Connectivity Influence Nutrient Retention in Tropical Rivers Receiving Wastewater Treatment Plant Discharge

  2021cites this paper
• Impacts of municipal wastewater treatment plant discharge on microbial community structure and function of the receiving river in Northwest Tibetan Plateau.

  2021cites this paper
• Determination of antibiotic resistance genes in a WWTP-impacted river in surface water, sediment, and biofilm: Influence of seasonality and water quality.

  2021cites this paper
• Monitoring Microbial Populations and Antibiotic Resistance Gene Enrichment Associated with Arctic Waste Stabilization Ponds

  2021cites this paper
• Additive Effects of Sediment and Nutrient on Leaf Litter Decomposition and Macroinvertebrates in Hyporheic Zone

  2021cites this paper
• Application of Omics Technologies for Microbial Community Structure and Function Analysis in Contaminated Environment

  2021cites this paper
• Contrasting community assembly processes structure lotic bacteria metacommunities along the river continuum

  2020cites this paper
• Decay of elevated antibiotic resistance genes in natural river sediments after sedimentation of wastewater particles.

  2020cites this paper
• Dynamics of coastal bacterial community average ribosomal RNA operon copy number reflect its response and sensitivity to ammonium and phosphate.

  2020cites this paper
• Functional Analysis of a Polluted River Microbiome Reveals a Metabolic Potential for Bioremediation

  2020cites this paper
• BALOs Improved Gut Microbiota Health in Postlarval Shrimp (Litopenaeus vannamei) After Being Subjected to Salinity Reduction Treatment

  2020cites this paper
• Chemometric Assessment of Bulgarian Wastewater Treatment Plants’ Effluents

  2020cites this paper
• Stable isotope ratios of emergent adult aquatic insects can be used as indicators of water pollution in the hyporheic food web

  2020cites this paper
• Fate of Trace Organic Compounds in Hyporheic Zone Sediments of Contrasting Organic Carbon Content and Impact on the Microbiome

  2020cites this paper
• Microbial Genes Involved in the Aromatic Compound Degradation Deciphered Through Metagenomics Analysis of Industrial Wastewater.

  2020cites this paper
• Effect of River Ecological Restoration on Biofilm

  2019influential citation
• Evaluating the Impact of Wastewater Effluent on Microbial Communities in the Panke, an Urban River

  2019cites this paper
• 16S rRNA gene based bacterial community structure of wastewater treatment plant effluents

  2019cites this paper
• Impact of wastewater effluent pollution on stream functioning: A whole-ecosystem manipulation experiment.

  2019cites this paper
• Antimicrobial-Resistant Escherichia coli from Environmental Waters in Northern Colorado

  2019cites this paper
• From genes to nitrogen removal: determining the impacts of poultry industry wastewater on tidal creek denitrification.

  2019cites this paper
• Profiling microbial communities in a watershed undergoing intensive anthropogenic activities.

  2019cites this paper
• Application of a novel molecular technique to characterise the effect of settling on microbial community composition of activated sludge.

  2019cites this paper
• Effect of River Ecological Restoration on Biofilm Microbial Community Composition

  2019cites this paper
• Physiological characteristics and toxin production of Microcystis aeruginosa (Cyanobacterium) in response to DOM in anaerobic digestion effluent.

  2019cites this paper
• Microbial assemblages and bioindicators as proxies for ecosystem health status: potential and limitations

  2019cites this paper
• Revealing chlorinated ethene transformation hotspots in a nitrate-impacted hyporheic zone.

  2019cites this paper
• Development of a mathematical model considering nutrients kinetics for assessing uMgeni river water quality.

  2018cites this paper
• Surveillance of antibiotic resistance determinants in Gaborone and Palapye wastewater treatment facilities

  2018cites this paper
• 16S RRNA-BASED ANALYSIS OF RIVER WATER AND SEDIMENT SAMPLES COLLECTED POST DISINFECTION AT CALUMET AND O’BRIEN WATER RECLAMATION PLANTS

  2018cites this paper
• Diagnosis and evaluation of an early-stage green bio-sorption reactor by life cycle assessment

  2018cites this paper
• Biodegradability of dissolved organic matter in Boom Clay pore water under nitrate-reducing conditions: Effect of additional C and P sources

  2018cites this paper
• River Bank Inducement Influence on a Shallow Groundwater Microbial Community and Its Effects on Aquifer Reactivity

  2018influential citation
• Inherent bacterial community response to multiple heavy metals in sediment from river-lake systems in the Poyang Lake, China.

  2018cites this paper
• Sewage Sludge Microbial Structures and Relations to Their Sources, Treatments, and Chemical Attributes

  2018cites this paper
• Gut bacterial assemblages of freshwater macroinvertebrate functional feeding groups

  2018cites this paper
• Identification of Degradation Pathways of Chlorohydrocarbons in Saturated Low-Permeability Sediments Using Compound-Specific Isotope Analysis.

  2018cites this paper
• Long-term industrial metal contamination unexpectedly shaped diversity and activity response of sediment microbiome.

  2018cites this paper
• Natural attenuation of chlorinated ethenes in hyporheic zones: A review of key biogeochemical processes and in-situ transformation potential.

  2018influential citation
• River biofilm community changes related to pharmaceutical loads emitted by a wastewater treatment plant

  2018cites this paper
• Toxicity evaluation of textile effluents and role of native soil bacterium in biodegradation of a textile dye

  2018cites this paper
• Microbial Community Response on Wastewater Discharge in Boreal Lake Sediments

  2017cites this paper
• Dehalococcoides as a Potential Biomarker Evidence for Uncharacterized Organohalides in Environmental Samples

  2017cites this paper
• Deciphering conjugative plasmid permissiveness in wastewater microbiomes

  2017influential citation
• Geochemical Parameters and Reductive Dechlorination Determine Aerobic Cometabolic vs Aerobic Metabolic Vinyl Chloride Biodegradation at Oxic/Anoxic Interface of Hyporheic Zones.

  2017cites this paper
• Changes in nitrogen-cycling microbial communities with depth in temperate and subtropical forest soils

  2017cites this paper
• Testing environmental controls on methane generation during microbial degradation of coal and oil from the Cherokee basin, Kansas

  2017cites this paper
• Removal of pharmaceutically active compounds in constructed wetlands: mechanisms and application

  2017cites this paper
• Unravelling riverine microbial communities under wastewater treatment plant effluent discharge in large urban areas

  2017cites this paper
• Natural attenuation of chlorinated ethenes in hyporheic zones : a review of 2 key biogeochemical processes and in-situ transformation potential 3

  2017influential citation
• The role of microbial diversity and composition in minimizing sludge production in the oxic-settling-anoxic process.

  2017cites this paper
• Impacts of antimicrobial compounds in urban waterways receiving sewer overflows

  2016cites this paper