Electrostatic interactions during electron transfer reactions between c-type cytochromes and flavodoxin.

The interaction of three different c-type cytochromes with flavodoxin has been studied by computer graphics modelling and computational methods. Flavodoxin and each cytochrome can make similar hypothetical electron transfer complexes that are characterized by nearly coplanar arrangement of the prosthetic groups, close intermolecular contacts at the protein-protein interface, and complementary intermolecular salt linkages. Computation of the electrostatic free energy of each complex showed that all were electrostatically stable. However, both the magnitude and behavior of the electrostatic stabilization as a function of solution ionic strength differed for the three cytochrome c-flavodoxin complexes. Variation in the computed electrostatic stabilization appears to reflect differences in the surface distribution of all charged groups in the complex, rather than differences localized at the site of intermolecular contact. The computed electrostatic association constants for the complexes and the measured kinetic rates of electron transfer in solution show a remarkable similarity in their ionic strength dependence. This correlation suggests electrostatic interactions influence electron transfer rates between protein molecules at the intermolecular association step. Comparative calculations for the three cytochrome c-flavodoxin complexes show that these ionic strength effects also involve all charged groups in both redox partners.

• Publication year

  1985

• Venue

  Journal of Biological Chemistry

• Publication date

  1985-05-10

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/S0021-9258(18)89060-3PMID 2985600
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Electrostatic effects in proteins.

  1985cited by this paper
• Electron transfer between flavodoxin semiquinone and c-type cytochromes: correlations between electrostatically corrected rate constants, redox potentials, and surface topologies.

  1984cited by this paper
• Electrostatic orientation during electron transfer between flavodoxin and cytochrome c

  1983cited by this paper
• Heme-heme orientation and electron transfer kinetic behavior of multisite oxidation-reduction enzymes.

  1983cited by this paper
• Structure of cytochrome c551 from Pseudomonas aeruginosa refined at 1.6 A resolution and comparison of the two redox forms.

  1982cited by this paper
• Anion binding and pH-dependent electrostatic effects in ribonuclease.

  1982cited by this paper
• Transient kinetics of electron transfer reactions of flavodoxin: ionic strength dependence of semiquinone oxidation by cytochrome c, ferricyanide, and ferric ethylenediaminetetraacetic acid and computer modeling of reaction complexes.

  1982cited by this paper
• Electrostatic effects in hemoglobin: electrostatic energy associated with allosteric transition and effector binding.

  1981cited by this paper
• Conformation change of cytochrome c. I. Ferrocytochrome c structure refined at 1.5 A resolution.

  1981cited by this paper
• Electrostatic effects in hemoglobin: hydrogen ion equilibria in human deoxy- and oxyhemoglobin A.

  1979cited by this paper
• The electric potential field around cytochrome c and the effect of ionic strength on reaction rates of horse cytochrome c.

  1978cited by this paper
• Structure of the semiquinone form of flavodoxin from Clostridum MP. Extension of 1.8 A resolution and some comparisons with the oxidized state.

  1978cited by this paper
• The Protein Data Bank: a computer-based archival file for macromolecular structures.

  1977cited by this paper
• Effect of modification of individual cytochrome c lysines on the reaction with cytochrome b5.

  1977cited by this paper
• Metalloprotein electron transfer reactions: analysis of reactivity of horse heart cytochrome c with inorganic complexes.

  1976cited by this paper
• The structure of oxidized cytochrome c 2 of Rhodospirillum rubrum.

  1976cited by this paper
• Electron transport by C-type cytochromes

  1975cited by this paper
• Electron transfer between biological molecules by thermally activated tunneling.

  1974cited by this paper
• Electrostatic effects in myoglobin. Hydrogen ion equilibria in sperm whale ferrimyoglobin.

  1974cited by this paper
• Structural bases for function in cytochromes c. An interpretation of comparative x-ray and biochemical data.

  1973cited by this paper
• The interpretation of protein structures: estimation of static accessibility.

  1971cited by this paper
• Chemical and Electrochemical Electron-Transfer Theory

  1964cited by this paper

• Electrostatic effects on water-soluble fullerene derivatives interaction with cytochrome c and cytochrome c oxidase

  2023cites this paper
• Transcriptomics analysis of the metabolic mechanisms of iron reduction induced by sulfate reduction mediated by sulfate-reducing bacteria.

  2021cites this paper
• Binding Mechanisms of Electron Transport Proteins with Cyanobacterial Photosystem I: An Integrated Computational and Experimental Model.

  2018cites this paper
• Characterisation of the electron transfer and complex formation between flavodoxin from D. vulgaris and the haem domain of cytochrome P450 BM3 from B. megaterium.

  2009cites this paper
• The role of the N(5) interaction and associated conformational changes in the modulation of the redox properties in flavoproteins

  2002cites this paper
• Gene Cluster of Rhodothermus marinusHigh-Potential Iron-Sulfur Protein:Oxygen Oxidoreductase, acaa3-Type Oxidase Belonging to the Superfamily of Heme-Copper Oxidases

  2001cites this paper
• Rational Design of P450 Enzymes for Biotechnology

  2001cites this paper
• Kinetic characterization of Desulfovibrio gigas hydrogenase upon selective chemical modification of amino acid groups as a tool for structure-function relationships.

  2000cites this paper
• Ionic strength dependence of the non-physiological electron transfer between flavodoxin and cytochrome c553 from D. vulgaris

  2000cites this paper
• In vitro and in vivo coupling of Thiocapsa hydrogenase with cyanobacterial and algal electron mediators.

  1999cites this paper
• Structure of a cytochrome P450-redox partner electron-transfer complex.

  1999cites this paper
• Cross-linking between cytochrome c3 and flavodoxin from Desulfovibrio gigas.

  1999cites this paper
• Structural and kinetic studies of the pyruvate-ferredoxin oxidoreductase/ferredoxin complex from Desulfovibrio africanus.

  1999cites this paper
• Effects of protein-protein interactions on electron transfer: docking and electron transfer calculations for complexes between flavodoxin and c-type cytochromes

  1999cites this paper
• NO news is good news.

  1998cites this paper
• Engineering multi-domain redox proteins containing flavodoxin as bio-transformer: preparatory studies by rational design.

  1998cites this paper
• Stopped-flow kinetic studies of low potential electron carriers of the photosynthetic bacterium, Rhodobacter capsulatus: ferredoxin I and NifF.

  1998cites this paper
• Escherichia coli flavodoxin sepharose as an affinity resin for cytochromes P450 and use to identify a putative cytochrome P450c17/3beta-hydroxysteroid dehydrogenase interaction.

  1997cites this paper
• Evaluation of the role of specific acidic amino acid residues in electron transfer between the flavodoxin and cytochrome c3 from Desulfovibrio vulgaris.

  1997cites this paper
• Comparison of low oxidoreduction potential cytochrome c553 from Desulfovibrio vulgaris with the class I cytochrome c family

  1996cites this paper
• [Electron transfer in hemoproteins. The applicability of models of electrostatic interaction of molecules for description of the relationship between the myoglobin and cytochrome reaction rate and the ionic strength].

  1996cites this paper
• Possible role of a short extra loop of the long-chain flavodoxin from Azotobacter chroococcum in electron transfer to nitrogenase: Complete 1H, 15N and 13C backbone assignments and secondary solution structure of the flavodoxin

  1996cites this paper
• Enzymatic and Electron Transfer Activities in Crystalline Protein Complexes (*)

  1996cites this paper
• Domain-domain interaction in cytochrome P450BM-3.

  1996cites this paper
• Electrostatic effects of surface acidic amino acid residues on the oxidation-reduction potentials of the flavodoxin from Desulfovibrio vulgaris (Hildenborough).

  1995cites this paper
• Thermodynamics of ferredoxin binding to ferredoxin:NADP+ reductase and the role of water at the complex interface.

  1994cites this paper
• A “parallel plate” electrostatic model for bimolecular rate constants applied to electron transfer proteins

  1994cites this paper
• Molecular Interactions Between Metalloproteins Involved in Electron Transfer Processes: Tetraheme Cytochrome c 3 and Flavodoxin. Nmr and Molecular Modeling Studies

  1994cites this paper
• Evidence for a ternary complex formed between flavodoxin and cytochrome c3: 1H-NMR and molecular modeling studies.

  1994cites this paper
• Flavodoxin and NADPH-flavodoxin reductase from Escherichia coli support bovine cytochrome P450c17 hydroxylase activities.

  1994cites this paper
• Involvement of electrostatic interactions in cytochrome c complex formations.

  1994cites this paper
• Laser flash photolysis studies of electron transfer to the cytochrome b5-cytochrome c complex.

  1993cites this paper
• Transient kinetics of electron transfer from a variety of c-type cytochromes to plastocyanin.

  1993cites this paper
• A hypothetical complex between crystalline flavocytochrome b2 and Cytochrome c

  1993cites this paper
• Voltammetric studies of the catalytic electron-transfer process between the Desulfovibrio gigas hydrogenase and small proteins isolated from the same genus.

  1993cites this paper
• Electron-transfer kinetics and electrostatic properties of the Rhodobacter sphaeroides reaction center and soluble c-cytochromes.

  1993cites this paper
• Brownian dynamics study of the influences of electrostatic interaction and diffusion on protein-protein association kinetics.

  1993cites this paper
• The major Kurloff cell glycoproteins: lectin affinities, glycosidase susceptibilities and relationship with the sialylated acid phosphatases of the Kurloff body.

  1992cites this paper
• Effect of KCl on the interactions between NADPH:cytochrome P-450 reductase and either cytochrome c, cytochrome b5 or cytochrome P-450 in octyl glucoside micelles.

  1992cites this paper
• Identification of specific carboxyl groups on Anabaena PCC 7119 flavodoxin which are involved in the interaction with ferredoxin-NADP+ reductase.

  1992cites this paper
• Electrostatic interactions stabilizing ferredoxin electron transfer complexes. Disruption by "conservative" mutations.

  1992cites this paper
• Dissociation of hydroxylase and lyase activities by site-directed mutagenesis of the rat P45017 alpha.

  1991cites this paper
• Unimolecular and bimolecular oxidoreduction reactions involving diprotein complexes of cytochrome c and plastocyanin. Dependence of electron-transfer reactivity on charge and orientation of the docked metalloproteins.

  1991cites this paper
• Molecular electrostatic potentials for characterizing drug-biosystem interactions.

  1991cites this paper
• Electrostatic orientation of the electron-transfer complex between plastocyanin and cytochrome c.

  1991cites this paper
• Studies on the flavin-binding region of old yellow enzyme with an active site probe, 8-fluoro-8-demethyl FMN.

  1991cites this paper
• Long-range electron transfer within metal-substituted protein complexes

  1991cites this paper
• PHOTOCHEMICAL INITIATION OF ELECTRON TRANSFER REACTIONS

  1991cites this paper
• Theoretical and empirical approaches to protein-structure prediction and analysis.

  1991cites this paper
• Isolation and characterization of the alkane-inducible NADPH-cytochrome P-450 oxidoreductase gene from Candida tropicalis. Identification of invariant residues within similar amino acid sequences of divergent flavoproteins.

  1990cites this paper
• Applications of interactive computer graphics in analyses of biomolecular structures.

  1990cites this paper
• Electrostatic effects on the spectral and redox properties of Clostridium pasteurianum flavodoxin: effects of salt concentration and polylysine.

  1990cites this paper
• Structural and chemical properties of a flavodoxin from Anabaena PCC 7119.

  1990cites this paper
• Molecular dynamics effects on protein electrostatics

  1989cites this paper
• Redox protein electron-transfer mechanisms: electrostatic interactions as a determinant of reaction site in c-type cytochromes.

  1989cites this paper
• Computational and site‐specific mutagenesis analyses of the asymmetric charge distribution on calmodulin

  1989cites this paper
• Mechanisms of flavoprotein-catalyzed reactions.

  1989cites this paper
• Electron Transfer Mechanisms in Heme Proteins

  1989cites this paper
• Laser flash photolysis as a probe of redox protein-membrane interactions: effect of binding of spinach plastocyanin and horse cytochrome c to lipid bilayer vesicles on the kinetics of reduction by flavin semiquinone.

  1989cites this paper
• Preparation and properties of a cross-linked complex between ferredoxin--NADP+ reductase and flavodoxin.

  1989cites this paper
• Electron transport in sulfate-reducing bacteria. Molecular modeling and NMR studies of the rubredoxin--tetraheme-cytochrome-c3 complex.

  1989cites this paper
• pKa values of the 8α-imidazole substituents in selected flavoenzymes containing 8α-histidylflavins☆

  1988cites this paper
• On the membrane topology of vertebrate cytochrome P-450 proteins.

  1988cites this paper
• Kinetics of the interaction of the cytochrome c oxidase of Paracoccus denitrificans with its own and bovine cytochrome c.

  1988cites this paper
• A hypothetical model of the flavodoxin-tetraheme cytochrome c3 complex of sulfate-reducing bacteria.

  1988cites this paper
• Model of a complex between the tetrahemic cytochrome c3 and the ferredoxin I from Desulfovibrio desulfuricans (Norway strain)

  1988cites this paper
• pKa values of the 8 alpha-imidazole substituents in selected flavoenzymes containing 8 alpha-histidylflavins.

  1988cites this paper
• Cytochrome c and c2 binding dynamics and electron transfer with photosynthetic reaction center protein and other integral membrane redox proteins.

  1988cites this paper
• Kinetic studies of electron transfer between hydrogenase and cytochrome c3 from Desulfovibrio gigas. Electrochemical properties of cytochrome c3

  1988cites this paper
• On the structure of old yellow enzyme studied by specific limited proteolysis.

  1987cites this paper
• Flavodoxin-cytochrome c interactions: circular dichroism and nuclear magnetic resonance studies.

  1987cites this paper
• Electrochemical study of the electron exchange between cytochrome c3 and hydrogenase from Desulfovibrio desulfuricans Norway.

  1987cites this paper
• Laser flash photolysis studies of electron transfer between ferredoxin-NADP+ reductase and several high-potential redox proteins.

  1987cites this paper
• Interaction of horse cytochrome c with the photosynthetic reaction center of Rhodospirillum rubrum.

  1987cites this paper
• Interaction of horse cytochromec with the photosynthetic reaction center ofRhodospirillum rubrum

  1987cites this paper
• Flavin radicals: chemistry and biochemistry.

  1987cites this paper
• Molecular dynamics of a cytochrome c-cytochrome b5 electron transfer complex.

  1987cites this paper
• Direct electrochemistry of bacterial cytochrome c551 at surface-modified gold electrodes

  1987cites this paper
• Reaction of cytochromes c and c2 with the Rhodobacter sphaeroides reaction center involves the heme crevice domain.

  1987cites this paper
• Elucidation of the factors which determine reaction-rate constants and biological specificity for electron-transfer proteins.

  1986cites this paper
• Electron-transfer reactions between flavodoxin semiquinone and c-type cytochromes: comparisons between various flavodoxins.

  1986cites this paper
• The modelling of electrostatic interactions in the function of globular proteins.

  1986cites this paper
• Reduction kinetics of the ferredoxin-ferredoxin-NADP+ reductase complex: a laser flash photolysis study.

  1986cites this paper
• HORSE HEART CYTOCHROME c AS AN ELECTRON ACCEPTOR FROM CHLOROPHYLL TRIPLET IN NEGATIVELY CHARGED LIPID BILAYER VESICLES *

  1986cites this paper
• Electrostatic analysis of the interaction of cytochrome c with native and dimethyl ester heme substituted cytochrome b5.

  1986cites this paper
• Kinetic studies of reduction of a 1:1 cytochrome c-flavodoxin complex by free flavin semiquinones and rubredoxin.

  1986cites this paper
• Distribution of ions around DNA, probed by energy transfer.

  1986cites this paper
• NADPH-cytochrome P-450 oxidoreductase: flavin mononucleotide and flavin adenine dinucleotide domains evolved from different flavoproteins.

  1986cites this paper
• Proton NMR study of the cytochrome c:flavodoxin electron transfer complex.

  1985cites this paper