Evaluation of fecal Activin-A as a novel biomarker for early diagnosis of ulcerative colitis using experimental murine animal model.

The present study was undertaken to evaluate fecal activin-A, in comparison with the currently used calprotectin, as a potential biomarker for early detection of ulcerative colitis (UC). Rats were divided into 4 groups (5 rats each). These included the acute UC group, where rats received 3% dextran sodium sulphate (DSS) for six consecutive days, and the chronic UC group, where rats received 3% DSS for three cycles (each cycle composed of 3 days of treatment and 12 days off). Control groups included animals that received tap water for six days and kept it as a negative control for short-term treatment and those received tap water for 45 days and served as a negative control for long-term treatment. Fecal samples were collected from all animal groups at the end of the study. Activin A and calprotectin levels were measured in collected fecal samples using a commercial ELISA kit for rat Calprotectin and rat Activin-A. The results revealed significant increases in fecal activin-A, and fecal calprotectin in chronic UC and a significant increase in fecal activin-A in acute colitis. It could be concluded that fecal activin-A is a useful biomarker for both acute and chronic UC. However, fecal calprotectin can be used as an indicator for chronic UC.

• Publication year

  2023

• Venue

  Assiut veterinary medical journal

• Publication date

  2023-07-03

• Fields of study

  Not labeled

• Identifiers
  DOI 10.21608/avmj.2023.198658.1130
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• Circulating S100A8/A9 is potentially a biomarker that could reflect the severity of experimental colitis in rats

  2020cited by this paper
• A Study on the Effects of Acetyl-11-Keto-β-Boswellic Acid Against Dextran Sodium Sulfate Induced Acute and Chronic Colitis in Swiss Albino Mice

  2020cited by this paper
• Biochemical evaluation and molecular docking assessment of the anti-inflammatory potential of Phyllanthus nivosus leaf against ulcerative colitis

  2020cited by this paper
• Galectin-1 reduces the severity of dextran sulfate sodium (DSS)-induced ulcerative colitis by suppressing inflammatory and oxidative stress response

  2020cited by this paper
• Apoptosis markers of circulating leukocytes are associated with the clinical course of inflammatory bowel disease

  2018cited by this paper
• An increased Bax/Bcl-2 ratio in circulating inflammatory cells predicts primary response to infliximab in inflammatory bowel disease patients

  2018cited by this paper
• Inducible nitric oxide synthase as a potential blood-based biomarker in inflammatory bowel diseases

  2018cited by this paper
• Fecal Calprotectin Levels Predict Histological Healing in Ulcerative Colitis

  2017cited by this paper
• Anti-ulcerative colitis activity of Calotropis procera Linn.

  2017cited by this paper
• Faecal and Serum Metabolomics in Paediatric Inflammatory Bowel Disease

  2016cited by this paper
• Autocrine pathways involving S100A8 and/or S100A9 that are postulated to regulate the immunological functions of macrophages in rats.

  2015cited by this paper
• The role and utility of faecal markers in inflammatory bowel disease

  2015cited by this paper
• Promising biological therapies for ulcerative colitis: A review of the literature.

  2015cited by this paper
• Disease Activity Assessment in IBD: Clinical Indices and Biomarkers Fail to Predict Endoscopic Remission

  2015cited by this paper
• Immunopathology of inflammatory bowel disease.

  2014cited by this paper
• A Review on Chemical-Induced Inflammatory Bowel Disease Models in Rodents

  2014cited by this paper
• Diagnostic Value of Fecal Calprotectin in Patient with Ulcerative Colitis

  2013cited by this paper
• Biomarkers in inflammatory bowel disease: current practices and recent advances.

  2012cited by this paper
• Ulcerative colitis: pathogenesis.

  2011cited by this paper
• The regulation and functions of activin and follistatin in inflammation and immunity.

  2011cited by this paper
• [Assessment of fecal calprotectin concentration as inflammatory marker in inflammatory bowel diseases in children--preliminary report].

  2010cited by this paper
• Faecal calprotectin for screening of patients with suspected inflammatory bowel disease: diagnostic meta-analysis

  2010cited by this paper
• Inflammation in atherosclerosis: transition from theory to practice.

  2010cited by this paper
• Monitoring disease activity by stool analyses: from occult blood to molecular markers of intestinal inflammation and damage

  2009cited by this paper
• Tetrandrine ameliorates dextran-sulfate-sodium-induced colitis in mice through inhibition of nuclear factor -κB activation

  2008cited by this paper
• Unravelling the pathogenesis of inflammatory bowel disease

  2007cited by this paper
• Myeloid-related proteins 8 and 14 induce a specific inflammatory response in human microvascular endothelial cells.

  2005cited by this paper
• The arachidonic acid‐binding protein S100A8/A9 promotes NADPH oxidase activation by interaction with p67phox and Rac‐2

  2005cited by this paper
• Relationship Between Fecal Calprotectin, Intestinal Inflammation, and Peripheral Blood Neutrophils in Patients with Active Ulcerative Colitis

  2004cited by this paper
• Potent induction of activin A secretion from monocytes and bone marrow stromal fibroblasts by cognate interaction with activated T cells

  2002cited by this paper
• Functional Relevance of Activin A in the Intestinal Epithelium

  2002cited by this paper
• Evidence for activin A and follistatin involvement in the systemic inflammatory response.

  2001cited by this paper
• Gene expression of activin, activin receptors, and follistatin in intestinal epithelial cells.

  2000cited by this paper
• Fecal calprotectin levels predict colorectal inflammation among patients with chronic diarrhea referred for colonoscopy

  2000cited by this paper
• Activin a modulates intestinal epithelial cell function in vitro

  2000cited by this paper
• Assessment of disease activity in ulcerative colitis by faecal calprotectin, a novel granulocyte marker protein.

  1997cited by this paper
• Activin A: a novel player and inflammatory marker in inflammatory bowel disease?

  1997cited by this paper

• Myostatin and Activin A as Biomarkers of Sarcopenia in Inflammatory Bowel Disease Patients

  2024cites this paper