Analysis of D-amino acid in Japanese post-fermented tea, Ishizuchi-kurocha

The D-amino acid content of Ishizuchi-kurocha, a post-fermented tea produced in Ehime, Japan, was measured. Ishizuchi-kurocha mainly contains D-glutamic acid and D-alanine, but it also contains a small amount of D-aspartic acid. Two types of lactic acid bacteria, Lactiplantibacillus plantarum and Levilactobacillus brevis, are the main species involved in lactic acid fermentation during the tea fermentation process. Therefore, the D-amino acid-producing abilities of strains of these two species isolated from Ishizuchi-kurocha were examined. Specifically, the production of D-aspartic acid, D-alanine, and D-glutamic acid by L. brevis and L. plantarum strains was observed. The amount of D-aspartic acid produced by L. plantarum was low. D-glutamine was detected in culture supernatant but not in bacterial cells. D-arginine was detected in bacterial cells of the L. plantarum strains but not in the culture supernatant. Both the L. brevis and L. plantarum strains possessed at least three kinds of putative racemase genes: alanine racemase, glutamate racemase, and aspartate racemase. However, their expression and enzyme activity remain unknown. L. plantarum and L. brevis could play an important role in the production of D-amino acids in Ishizuchi-kurocha. In fact, Ishizuchi-kurocha is expected to possess the effective physiological activities of D-amino acids.

• Publication year

  2023

• Venue

  Bioscience of Microbiota Food and Health

• Publication date

  2023-06-28

• Fields of study

  Agricultural and Food Sciences, Medicine

• Identifiers
  DOI 10.12938/bmfh.2023-005PMID 37791341PMCID 10542427
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Suppressive effects of D-amino acids on the lipid accumulation in human hepatocyte.

  2022cited by this paper
• d-Amino Acids and pLG72 in Alzheimer’s Disease and Schizophrenia

  2021cited by this paper
• d-Serine Mediates Cellular Proliferation for Kidney Remodeling

  2021cited by this paper
• Serum d-serine levels are altered in early phases of Alzheimer’s disease: towards a precocious biomarker

  2021cited by this paper
• Draft Genome Sequence of Lactobacillus plantarum IYO1511, Isolated from Ishizuchi-Kurocha

  2020cited by this paper
• d-Amino Acids and Lactic Acid Bacteria

  2019cited by this paper
• Evaluation of lactic acid bacteria and component change during fermentation of Ishizuchi‐kurocha

  2019cited by this paper
• Regional characteristics of Lactobacillus plantarum group strains isolated from two kinds of Japanese post-fermented teas, Ishizuchi-kurocha and Awa-bancha

  2018cited by this paper
• Gut microbiota-derived D-serine protects against acute kidney injury.

  2018cited by this paper
• Free D-amino acids produced by commensal bacteria in the colonic lumen

  2018cited by this paper
• Combination probiotics may prevent Clostridium difficile infection among elderly patients undergoing an orthopedic surgery

  2018cited by this paper
• A Novel Bifunctional Amino Acid Racemase With Multiple Substrate Specificity, MalY From Lactobacillus sakei LT-13: Genome-Based Identification and Enzymological Characterization

  2018cited by this paper
• Comparison of antioxidant activities among four kinds of Japanese traditional fermented tea

  2016cited by this paper
• "D-Amino acid", a New Ingredient Effecting on Sake Taste

  2015cited by this paper
• Distribution of D-amino acids in vinegars and involvement of lactic acid bacteria in the production of D-amino acids

  2013cited by this paper
• Plasma levels of D-serine in Brazilian individuals with schizophrenia.

  2012cited by this paper
• New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0.

  2010cited by this paper
• A periplasmic, pyridoxal-5′-phosphate-dependent amino acid racemase in Pseudomonas taetrolens

  2009cited by this paper
• D-Serine Is the Dominant Endogenous Coagonist for NMDA Receptor Neurotoxicity in Organotypic Hippocampal Slices

  2005cited by this paper
• Free D-aspartate in mammals.

  2005cited by this paper
• The role of D-aspartic acid and N-methyl-D-aspartic acid in the regulation of prolactin release.

  2000cited by this paper
• D-aspartate modulates melatonin synthesis in rat pinealocytes.

  1998cited by this paper
• The presence of free D‐serine in rat brain

  1992cited by this paper
• [Antibacterial and anti-hemolysin activities of tea catechins and their structural relatives].

  1990cited by this paper
• Arginine racemase of Pseudomonas graveolens. I. Purification, crystallization, and properties.

  1971cited by this paper

• Differences and correlations between chiral amino acid contents and microbiota in different processing stages of Keemun black tea.

  2026cites this paper
• Gut Barrier Dysfunction and Microbiota Variations in Cryptosporidiosis: A Comprehensive Review

  2025cites this paper
• Constituent-taste relationship of Kuding tea fermented by Aspergillus neoniger and Aspergillus cristatus: Unveiling taste characteristics through untargeted metabolomics

  2024cites this paper
• Analysis of lactic acid bacteria species in Miang, a post-fermented tea in Thailand, and their potential use as probiotics

  2024cites this paper
• Effects of different fermentation modes on tea leaves: revealing the metabolites modification by quasi-targeted metabolomics

  2024cites this paper
• Screening and Identification of Natural Compounds as Potential Inhibitors of Glutamate Racemase and an Emerging Drug Target of Food Pathogen E. coli O157:H7: An In silico Approach to Combat Increasing Drug Resistance.

  2024cites this paper
• Relationship between the Physiological Activity of Japanese Post-Fermented Teas and Lactic Acid Bacteria

  2023cites this paper