High-performance optical control of GPCR signaling by bistable animal opsins MosOpn3 and LamPP in a molecular property–dependent manner

Significance Optogenetics for GPCR signaling is highly valuable but is still developing and requires effective tools like ChR2. We demonstrated that two animal opsins, mosquito Opn3 (MosOpn3) and lamprey parapinopsin (LamPP), are available in optogenetics equivalently to ChR2 in terms of retinal requirement. Furthermore, MosOpn3 introduced into nociceptor neurons of C. elegans exhibited ~7,000 times higher sensitivity than ChR2 in the light-induced avoidance behavior. LamPP introduced into motor neurons induced violet light-dependent stop and green light-dependent go, demonstrating color-dependent control of behavior using LamPP. In addition, our molecular engineering extended the usability of MosOpn3 and LamPP to different signaling cascades and kinetics. The current findings provide numerous strategies for optical control of various GPCR-based physiologies as well as GPCR signaling itself.

• Publication year

  2022

• Venue

  Proceedings of the National Academy of Sciences of the United States of America

• Publication date

  2022-11-23

• Fields of study

  Biology, Medicine, Physics, Engineering

• Identifiers
  DOI 10.1073/pnas.2204341119PMID 36417444PMCID 9889881
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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