Harvesting mouse suprachiasmatic nucleus by vibrating microtome for diurnal transcriptome analysis

Summary The mammalian suprachiasmatic nucleus (SCN) is the principal circadian clock that synchronizes daily behavioral and physiological responses in response to environmental cues. Here, we present a protocol for harvesting mouse SCN by vibrating microtome for diurnal transcriptome analysis. We describe steps for mouse entrainment, isolation of the SCN, tissue preparation, slicing with a vibratome, and handling of the harvested SCN for RNA extraction. This protocol can also be used for harvesting other mammalian brain regions for genomic studies.

• Publication year

  2023

• Venue

  STAR Protocols

• Publication date

  2023-09-26

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/j.xpro.2023.102618PMID 37756154PMCID 10539961
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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