pALEX, a dual-tag prokaryotic expression vector for the purification of full-length proteins.

pALEX, a prokaryotic expression vector, was constructed in which the multiple cloning site (MCS, polylinker) is flanked by sequences encoding glutathione S-transferase (GST) at the 5' end and a His6 residue tag at the 3' end. Open reading frames cloned into this vector can direct production of fusion proteins with GST at their N terminus and a His6 tag at their C terminus. This allows for the purification of full-size fusion proteins by a sequential two-step procedure on glutathione-agarose and Ni(2+)-agarose columns.

• Publication year

  1995

• Venue

  Gene

• Publication date

  1995-10-16

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/0378-1119(95)00417-5PMID 7590319
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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