Effect of Bushen Huoxue recipe on serum metabolomics in polycystic ovary syndrome rats

Abstract Objective To investigate the effect of Bushen Huoxue Recipe (BSHXR) on serum metabolomics in polycystic ovary syndrome rat (PCOSR). Methods In our study, twenty-four 6-week-old Sprague-Dawley (SD) female rats were randomly divided into three groups: treatment group, model group and blank group. The blank group and other groups were gavaged in different ways each morning, and the rats were treated with normal saline or BSHXR containing liquid each afternoon. Liquid chromatography-mass spectrometry (LC-MS) was employed to study serum metabolites in the treatment group after the study as well as in the model and blank groups. Results There was a tendency to normalize the histomorphology of ovarian pathology and the abnormal sex hormone level of PCOSR was significantly improved after BSHXR treatment. The level of serum metabolites was greatly changed in PCOSR treated with the BSHXR. We identified 32 metabolic targets of BSHXR in PCOSR using LC-MS, and further revealed BSHXR targeted five major metabolic pathway: retrograde endocannabinoid signaling, taurine and hypotaurine metabolism, glycerophospholipid metabolism, primary bile acid biosynthesis, arginine and proline metabolism. Conclusion: Our study found that BSHXR plays a role in the treatment of PCOS by regulating key metabolic pathways in the PCOSR.

• Publication year

  2023

• Venue

  Gynecological Endocrinology

• Publication date

  2023-10-17

• Fields of study

  Medicine

• Identifiers
  DOI 10.1080/09513590.2023.2260500PMID 37849277
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Lipid metabolism and endometrial receptivity.

  2022cited by this paper
• Association of Hormonal Contraceptive Use With Adverse Health Outcomes

  2022cited by this paper
• Phosphatidylserine enhances anti‐inflammatory effects of reconstituted HDL in macrophages via distinct intracellular pathways

  2022cited by this paper
• Changes in the prevalence of polycystic ovary syndrome in China over the past decade

  2022cited by this paper
• Analysis of 2-Arachidonoylglycerol Levels in Polycystic Ovary Syndrome in the Context of Hormonal and Metabolic Alterations and Across the Classical Phenotypes

  2022cited by this paper
• Effects of increasing intake of soybean oil on synthesis of testosterone in Leydig cells

  2021cited by this paper
• Polycystic ovary syndrome and cardiometabolic risk: Opportunities for cardiovascular disease prevention.

  2020cited by this paper
• Dynamic Analysis of the Biochemical Changes in Rats with Polycystic Ovary Syndrome (PCOS) Using Urinary 1H NMR-Based Metabonomics

  2020cited by this paper
• Androgens increase accumulation of advanced glycation end products in granulosa cells by activating ER stress in PCOS.

  2020cited by this paper
• The role of androgen and its related signals in PCOS

  2020cited by this paper
• Downregulation of MicroRNA-222 Reduces Insulin Resistance in Rats with PCOS by Inhibiting Activation of the MAPK/ERK Pathway via Pten

  2020cited by this paper
• Guizhi Fuling Wan, Chinese Herbal Medicine, Ameliorates Insulin Sensitivity in PCOS Model Rats With Insulin Resistance via Remodeling Intestinal Homeostasis

  2020cited by this paper
• Animal Models to Understand the Etiology and Pathophysiology of Polycystic Ovary Syndrome

  2020cited by this paper
• Increased circulating conjugated primary bile acids are associated with hyperandrogenism in women with polycystic ovary syndrome.

  2019cited by this paper
• Changes in whole metabolites after exenatide treatment in overweight/obese polycystic ovary syndrome patients

  2019cited by this paper
• Assessment of follicular fluid metabolomics of polycystic ovary syndrome in kidney yang deficiency syndrome

  2019cited by this paper
• Another piece in the polycystic ovary syndrome-depression/anxiety puzzle: body image.

  2019cited by this paper
• Metabolomics 20 years on: what have we learned and what hurdles remain?

  2018cited by this paper
• Ten‐year weight gain is associated with elevated fasting insulin levels and precedes glucose elevation

  2018cited by this paper
• Hepatic De Novo Lipogenesis and Regulation of Metabolism

  2016cited by this paper
• Endocannabinoid receptor blockade increases hepatocyte growth factor and reduces insulin levels in obese women with polycystic ovary syndrome

  2016cited by this paper
• The effect of L-arginine and ascorbic acid on the visceral fat and the concentrations of metalloproteinases 2 and 9 in high-fat-diet rats.

  2015cited by this paper
• Endocannabinoid system activation may be associated with insulin resistance in women with polycystic ovary syndrome.

  2015cited by this paper
• Mitochondrial dysfunction and insulin resistance: an update

  2014cited by this paper
• Current practice of liquid chromatography-mass spectrometry in metabolomics and metabonomics.

  2014cited by this paper
• Reporting the rates of depression in polycystic ovary syndrome (PCOS).

  2014cited by this paper
• Increased prolidase activity and oxidative stress in PCOS

  2013cited by this paper
• Mechanism of Vitamin D Receptor Inhibition of Cholesterol 7α-Hydroxylase Gene Transcription in Human Hepatocytes

  2009cited by this paper
• The Bile Acid Synthesis Pathway Is Present and Functional in the Human Ovary

  2009cited by this paper
• Farnesoid X Receptor, through the Binding with Steroidogenic Factor 1-responsive Element, Inhibits Aromatase Expression in Tumor Leydig Cells*

  2009cited by this paper
• Dietary L-arginine supplementation reduces white fat gain and enhances skeletal muscle and brown fat masses in diet-induced obese rats.

  2009cited by this paper
• Cannabinoid receptors 1 and 2 (CB1 and CB2), their distribution, ligands and functional involvement in nervous system structures--a short review.

  2008cited by this paper
• Presence of functional cannabinoid receptors in human endocrine pancreas

  2008cited by this paper
• Dual effects of nitric oxide on meiotic maturation of mouse cumulus cell-enclosed oocytes in vitro.

  2003cited by this paper
• Invertebrate molecular neuroimmune processes.

  2000cited by this paper
• Identification of a nuclear receptor for bile acids.

  1999cited by this paper

• Exploring the mechanism of action of aspirin in improving endometrial receptivity in PCOS rats based on uterine lavage fluid metabolomics

  2025cites this paper
• Investigating the Therapeutic Effects of Bushen Huoxue Formula Combined with Aerobic Exercise on Reproductive Function in Obese Polycystic Ovary Syndrome: An Experimental Study Based on the Integration of Sports and Medicine

  2025cites this paper
• Insight into metabolic dysregulation of polycystic ovary syndrome utilizing metabolomic signatures: a narrative review

  2024cites this paper