BackgroundEukaryotic organisms are defined by the presence of a nucleus, which encloses the chromosomal DNA, and is characterized by its DNA content (C-value). Complex eukaryotic organisms contain organs and tissues that comprise interspersions of different cell types, within which polysomaty, endoreduplication, and cell cycle arrest is frequently observed. Little is known about the distribution of C-values across different cell types within these organs and tissues.ResultsWe have developed, and describe here, a method to precisely define the C-value status within any specific cell type within complex organs and tissues of plants. We illustrate the application of this method to Arabidopsis thaliana, specifically focusing on the different cell types found within the root.ConclusionThe method accurately and conveniently charts C-value within specific cell types, and provides novel insight into developmental processes. The method is, in principle, applicable to any transformable organism, including mammals, within which cell type specificity of regulation of endoreduplication, of polysomaty, and of cell cycle arrest is suspected.
Cell type-specific characterization of nuclear DNA contents within complex tissues and organs
Changqing Zhang,Fangcheng Gong,G. Lambert,David W. Galbraith
Published 2005 in Plant Methods
ABSTRACT
PUBLICATION RECORD
- Publication year
2005
- Venue
Plant Methods
- Publication date
2005-10-04
- Fields of study
Biology, Medicine
- Identifiers
- External record
- Source metadata
Semantic Scholar, PubMed
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EXTRACTION MAP
CLAIMS
CONCEPTS
- arabidopsis thaliana
A model flowering plant used as the example organism in the root tissue application.
Aliases: A. thaliana, Arabidopsis
- cell cycle arrest
A state in which cell-cycle progression stops or pauses in a cell type.
- cell type-specific characterization
An approach that assigns a measurement or trait to individual cell types within a mixed tissue.
Aliases: cell type-specific definition
- c-value status
The DNA-content state of a nucleus or cell, used here to describe specific cell types.
Aliases: C-value
- endoreduplication
A cell-cycle variant in which DNA is replicated without cell division, increasing nuclear DNA content.
Aliases: endoreduplication cycle
- polysomaty
The presence of cells or nuclei with different ploidy levels within the same tissue.
- root cell types
The distinct cell populations present in the root of Arabidopsis thaliana.
Aliases: different cell types found within the root
- transformable organism
An organism that can be genetically transformed, which is the class of systems the method is said to suit in principle.
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