Isolation and separation of highly enriched fractions of viable mouse gastric parietal cells by velocity sedimentation

Methods of tissue dissociation and cell separation have been modified to obtain highly enriched fractions of mouse gastric parietal cells. Suspension of gastric mucosal cells are prepared by pronase digestion of the glandular portion of the stomach from adult mice. By utilizing the velocity sedimentation technique to separate cells of different sizes it is possible to recovery parietal cells, which are larger than the other cell types, in fractions with purity of 75-95%. The homogeneity of cell fractions has been assessed by light and electron microscopy. The ability of the isolated cells to exclude the dye trypan blue, to incorporate labeled substrate, to consume oxygen, and to retain their structural integrity indicates that they are viable and still capable of functional activity.

• Publication year

  1975

• Venue

  Journal of Cell Biology

• Publication date

  1975-05-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1083/JCB.65.2.428PMID 1127018PMCID 2109418
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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