Histone Deacetylase 2 Stabilizes SPARC-related Modular Calcium Binding 2 to Promote Metastasis and Stemness in Gallbladder Cancer.

BACKGROUND We aimed to investigate the relationship between histone deacetylase 2 (HDAC2) and SPARC-related modular calcium binding 2 (SMOC2) and the role of SMOC2 in gallbladder cancer (GBC). METHODS The expression of HDAC2 and SMOC2 in GBC and normal cells was detected by quantitative real-time reverse transcription polymerase chain reaction (qRTPCR), which was also used to detect the mRNA stability of SMOC2. The combination between HDAC2 and SMOC2 was detected by Chromatin immunoprecipitation (ChIP) assay. After silencing and/or overexpressing HDAC2 and SMOC2, cell viability, migration, invasion, and stemness were respectively tested by the Cell Counting Kit-8 (CCK-8), cell scratch, transwell, and sphere-formation assay. RESULTS In GBC cells, HDAC2 and SMOC2 were highly expressed. HDAC2 combined with SMOC2 promoted mRNA stability of SMOC2. HDAC2 or SMOC2 overexpression promoted GBC cell metastasis and stemness. SMOC2 overexpression rescued the negative effects of silencing HDAC2 in GBC. CONCLUSION HDAC2 stabilizes SMOC2 to promote metastasis and stemness in gallbladder cancer.

• Publication year

  2024

• Venue

  Current molecular medicine

• Publication date

  2024-01-03

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.2174/0115665240257970231013094101PMID 38173203
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Roles of HDAC2, eIF5, and eIF6 in Lung Cancer Tumorigenesis

  2021cited by this paper
• TGF-β1/SMOC2/AKT and ERK axis regulates proliferation, migration, and fibroblast to myofibroblast transformation in lung fibroblast, contributing with the asthma progression

  2021cited by this paper
• Smoc1 and Smoc2 regulate bone formation as downstream molecules of Runx2

  2021cited by this paper
• HDAC2 interacts with microRNA-503-5p to regulate SGK1 in osteoarthritis

  2021cited by this paper
• Histone Deacetylase 2 selective inhibitors: A versatile therapeutic strategy as next generation drug target in cancer therapy.

  2021cited by this paper
• Cell Stemness, Epithelial-to-Mesenchymal Transition, and Immunoevasion: Intertwined Aspects in Cancer Metastasis.

  2020cited by this paper
• HDAC2 inhibits EMT-mediated cancer metastasis by downregulating the long noncoding RNA H19 in colorectal cancer

  2020cited by this paper
• HDAC2‐mediated proliferation of trophoblast cells requires the miR‐183/FOXA1/IL‐8 signaling pathway

  2020cited by this paper
• A SMOC2 variant inhibits BMP signaling by competitively binding to BMPR1B and causes growth plate defects.

  2020cited by this paper
• SMOC2, an intestinal stem cell marker, is an independent prognostic marker associated with better survival in colorectal cancers

  2020cited by this paper
• Downregulation of SMOC2 expression in papillary thyroid carcinoma and its prognostic significance

  2020cited by this paper
• Gallbladder and extrahepatic bile duct cancers in the Americas: Incidence and mortality patterns and trends

  2020cited by this paper
• Nanomedicine‐Based Immunotherapy for the Treatment of Cancer Metastasis

  2019cited by this paper
• HDAC2 depletion promotes osteosarcoma’s stemness both in vitro and in vivo: a study on a putative new target for CSCs directed therapy

  2018cited by this paper
• Histone deacetylase 3 is associated with gastric cancer cell growth via the miR-454-mediated targeting of CHD5

  2017cited by this paper
• Histone deacetylase 11 suppresses p53 expression in pituitary tumor cells

  2017cited by this paper
• New Horizons for Precision Medicine in Biliary Tract Cancers.

  2017cited by this paper
• Silencing SMOC2 ameliorates kidney fibrosis by inhibiting fibroblast to myofibroblast transformation.

  2017cited by this paper
• JunB promotes cell invasion, migration and distant metastasis of head and neck squamous cell carcinoma

  2016cited by this paper
• Smoc2 potentiates proliferation of hepatocellular carcinoma cells via promotion of cell cycle progression

  2016cited by this paper
• HDAC1 promoted migration and invasion binding with TCF12 by promoting EMT progress in gallbladder cancer

  2016cited by this paper
• Sox2/Oct4: A delicately balanced partnership in pluripotent stem cells and embryogenesis.

  2016cited by this paper
• Induction of the intestinal stem cell signature gene SMOC-2 is required for L1-mediated colon cancer progression

  2016cited by this paper
• An Arntl2-Driven Secretome Enables Lung Adenocarcinoma Metastatic Self-Sufficiency.

  2016cited by this paper
• Histone deacetylase 6 in health and disease.

  2015cited by this paper
• Matricellular proteins: multifaceted extracellular regulators in tumor dormancy

  2014cited by this paper
• Revisiting the matricellular concept

  2014cited by this paper
• MicroRNA-Regulated Protein-Protein Interaction Networks and Their Functions in Breast Cancer

  2013cited by this paper
• Matricellular proteins: priming the tumour microenvironment for cancer development and metastasis

  2013cited by this paper
• Histone deacetylases activate hepatocyte growth factor signaling by repressing microRNA-449 in hepatocellular carcinoma cells.

  2012cited by this paper
• The Lgr5 intestinal stem cell signature: robust expression of proposed quiescent ‘+4’ cell markers

  2012cited by this paper
• Emerging functions of matricellular proteins

  2011cited by this paper
• HDAC2 promotes cell migration/invasion abilities through HIF-1α stabilization in human oral squamous cell carcinoma.

  2011cited by this paper
• Genome-Wide Analysis of Promoter Methylation Associated with Gene Expression Profile in Pancreatic Adenocarcinoma

  2011cited by this paper
• SIRT3 is a mitochondria-localized tumor suppressor required for maintenance of mitochondrial integrity and metabolism during stress.

  2010cited by this paper
• HDAC2: a critical factor in health and disease.

  2009cited by this paper
• Expression microarray analysis of papillary thyroid carcinoma and benign thyroid tissue: emphasis on the follicular variant and potential markers of malignancy

  2007cited by this paper
• Identification of candidate methylation-responsive genes in ovarian cancer

  2007cited by this paper
• Stress-Dependent Regulation of FOXO Transcription Factors by the SIRT1 Deacetylase

  2004cited by this paper
• Histone deacetylases (HDACs): characterization of the classical HDAC family.

  2003cited by this paper
• Targeted cellular process profiling approach for uterine leiomyoma using cDNA microarray, proteomics and gene ontology analysis

  2003cited by this paper
• DNA methyltransferase Dnmt1 associates with histone deacetylase activity

  2000cited by this paper

• SMOC2 promotes peritoneal metastasis of gastric cancer involving Wnt/β-catenin pathway modulation and inducing angiogenesis

  2025cites this paper