Challenging the phylogenetic relationships among Echinococcus multilocularis isolates from main endemic areas.

Alveolar echinococcosis (AE) is a rare but severe disease that affects more than 18,000 people worldwide per year. The complete sequencing of the mitochondrial genome of Echinococcus multilocularis has made it possible to study the genetic diversity of the parasite and its spatial and temporal evolution. We amplified the whole mitochondrial genome by PCR, using one uniplex and two multiplex reactions to cover the 13,738 bp of the mitogenome, and then sequenced the amplicons with Illumina technology. In total, 113 samples from Europe, Asia, , the Arctic and North America were analyzed. Three major haplogroups were found: HG1, which clustered samples from Alaska (including Saint-Lawrence Island), Yakutia (Russia) and Svalbard; HG2, with samples from Asia, North America and Europe; and HG3, subdivided into three micro-haplogroups. HG3a included samples from North America and Europe, whereas HG3b and HG3c only include samples from Europe. In France, HG3a included samples from patients more recently diagnosed in a region outside the historical endemic area. A fourth putative haplogroup, HG4, was represented by only one isolate from Olkhon Island (Russia). The increased discriminatory power of the complete sequencing of the E. multilocularis mitogenome has made it possible to highlight four distinct geographical clusters, one being divided into three micro-haplogroups in France.

• Publication year

  2024

• Venue

  International Journal of Parasitology

• Publication date

  2024-05-01

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1016/j.ijpara.2024.05.004PMID 38815855
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  2013cited by this paper
• Genetic diversity of Echinococcus spp. in Russia

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• The impact of globalisation on the distribution of Echinococcus multilocularis.

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• Integrative Genomics Viewer (IGV): high-performance genomics data visualization and exploration

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• EmsB, a tandem repeated multi-loci microsatellite, new tool to investigate the genetic diversity of Echinococcus multilocularis.

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• A molecular phylogeny of the genus Echinococcus inferred from complete mitochondrial genomes

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• Mitochondrial DNA and human evolution.

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• The incomplete natural history of mitochondria

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• Biological, Epidemiological, and Clinical Aspects of Echinococcosis, a Zoonosis of Increasing Concern

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• [Comparison observation on the mature alveolar of Echinococcus sibiricensis and Echinococcus multilocularis in the experimentally infected white mice].

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• Transberingian dispersal of cestodes in mammals.

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• Statistical method for testing the neutral mutation hypothesis by DNA polymorphism.

  1989cited by this paper
• [Echinococcus multiocularis in South Germany. I. The tapeworm stage of strains from humans and animals].

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• Studies on the helminth fauna of Alaska. XXIV. Echinococcus sibiricensis n. sp., from St. Lawrence Island.

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