Frequency of virulence genes in Escherichia coli strains isolated from piglets with diarrhea in the North Parana State, Brazil

Identification of Escherichia coli causing porcine postweaning diarrhea requires knowledge regarding the prevalent pathotypes within a given region. A total of 100 Escherichia coli isolates from piglets with diarrhea in Londrina city, Parana State, South Brazil, were screened for the presence of genes for F4, F5, F6, F18, F41 fimbrial antigens by specific probes and for enterotoxins (STa, STb, LT and STx2e) by polymerase chain reaction (PCR). The results showed that 60% of the isolates were positive for one or more of the fimbrial antigens and 92% were positive at least for one of the virulence factors examined. Virulence factor genes detected were F4 (44%), F18 (38%), F5 (30%), F41 (32%), F6 (25%), LTp-I (71%), STa (40%), STb (47%) and STx2e (3%). Twenty four patterns of virulence factor according to the different virulence genes form were found and the most frequent virulence gene pattern was F4, F18, F41, STa, STb and LT. Most of the isolates that carried genes for adhesins also harboured genes for toxins.

• Publication year

  2009

• Venue

  Brazilian Journal of Microbiology

• Publication date

  2009-03-01

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1590/S1517-838220090001000035PMID 24031344PMCID 3768501
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  2007influential reference
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  2006influential reference
• Pathotypes and serogroups of enterotoxigenic Escherichia coli isolated from pre-weaning pigs in north Vietnam.

  2006cited by this paper
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• Rapid identification of virulence genes in enterotoxigenic Escherichia coli isolates associated with diarrhoea in Queensland piggeries.

  2005cited by this paper
• Enterotoxigenic Escherichia coli in veterinary medicine.

  2005influential reference
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  2005influential reference
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  2002influential reference
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  2002influential reference
• Pathogenic characteristics of Escherichia coli strains isolated from newborn piglets with diarrhea in Brazil.

  2000cited by this paper
• Digoxigenin-labelled probe for the detection of enteropathogenic Escherichia coli

  1998cited by this paper
• Colonization antigens of enterotoxigenic Escherichia coli strains isolated from piglets in Spain.

  1997cited by this paper
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  1997influential reference
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  1995influential reference
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• Evolutionary origin of pathogenic determinants in enterotoxigenic Escherichia coli and Vibrio cholerae O1

  1987cited by this paper
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  1969cited by this paper

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