Insertion of Endocellulase Catalytic Domains into Thermostable Consensus Ankyrin Scaffolds: Effects on Stability and Cellulolytic Activity

No abstract is available for this paper.

• Publication year

  2013

• Venue

  Applied and Environmental Microbiology

• Publication date

  2013-08-23

• Fields of study

  Biology, Materials Science, Chemistry, Environmental Science, Medicine

• Identifiers
  DOI 10.1128/AEM.02121-13PMID 23974146PMCID PMC3811507
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Engineering domain-swapped binding interfaces by mutually exclusive folding.

  2012cited by this paper
• In Vitro Reconstitution of the Complete Clostridium thermocellum Cellulosome and Synergistic Activity on Crystalline Cellulose

  2012cited by this paper
• Enhanced activity of Thermotoga maritima cellulase 12A by mutating a unique surface loop

  2012cited by this paper
• Crystal structure and substrate‐binding mode of cellulase 12A from Thermotoga maritima

  2011cited by this paper
• The contribution of entropy, enthalpy, and hydrophobic desolvation to cooperativity in repeat-protein folding.

  2011cited by this paper
• Enzymatic hydrolysis of cellulosic biomass

  2011cited by this paper
• Engineering of Clostridium phytofermentans Endoglucanase Cel5A for Improved Thermostability

  2010cited by this paper
• With contribution from

  2010cited by this paper
• Analysis of repeat-protein folding using nearest-neighbor statistical mechanical models.

  2009influential reference
• The rosettazyme: a synthetic cellulosome.

  2009cited by this paper
• Enzyme stabilization by domain insertion into a thermophilic protein.

  2009cited by this paper
• Effect of interdomain linker length on an antagonistic folding-unfolding equilibrium between two protein domains.

  2009cited by this paper
• Solvent denaturation of proteins and interpretations of the m value.

  2009cited by this paper
• Rerouting the folding pathway of the Notch ankyrin domain by reshaping the energy landscape.

  2008cited by this paper
• Folding and unfolding mechanism of highly stable full-consensus ankyrin repeat proteins.

  2008cited by this paper
• The Carbohydrate-Active EnZymes database (CAZy): an expert resource for Glycogenomics

  2008cited by this paper
• Stabilizing ionic interactions in a full-consensus ankyrin repeat protein.

  2008cited by this paper
• A transition from cellulose swelling to cellulose dissolution by o-phosphoric acid: evidence from enzymatic hydrolysis and supramolecular structure.

  2006cited by this paper
• High-throughput screening for enhanced protein stability.

  2006cited by this paper
• Environmental, economic, and energetic costs and benefits of biodiesel and ethanol biofuels.

  2006cited by this paper
• Breaking the Biological barriers to Cellulosic Ethanol: A Joint Research Agenda

  2006cited by this paper
• Breaking the Biological Barriers to Cellulosic Ethanol: A Joint Research Agenda

  2006cited by this paper
• Protein production by auto-induction in high density shaking cultures.

  2005cited by this paper
• Structural and biochemical studies of GH family 12 cellulases: improved thermal stability, and ligand complexes.

  2005cited by this paper
• Action of Designer Cellulosomes on Homogeneous Versus Complex Substrates

  2005cited by this paper
• The cellulosomes: multienzyme machines for degradation of plant cell wall polysaccharides.

  2004cited by this paper
• Three-parameter lognormal distribution ubiquitously found in cDNA microarray data and its application to parametric data treatment

  2004cited by this paper
• HMM Logos for visualization of protein families

  2004cited by this paper
• Activity-Stability Relationships in Extremophilic Enzymes*

  2003cited by this paper
• Structure and stability of the ankyrin domain of the Drosophila Notch receptor

  2003cited by this paper
• Activity, stability and flexibility in glycosidases adapted to extreme thermal environments.

  2003cited by this paper
• Comparison of family 12 glycoside hydrolases and recruited substitutions important for thermal stability

  2003cited by this paper
• Carbohydrate-induced Differential Gene Expression Patterns in the Hyperthermophilic Bacterium Thermotoga maritima * 210

  2003cited by this paper
• Degradation of Cellulose Substrates by Cellulosome Chimeras

  2002cited by this paper
• Biochemical characterization of Thermotoga maritima endoglucanase Cel74 with and without a carbohydrate binding module (CBM)

  2002cited by this paper
• Hydrolysis of lignocellulosic materials for ethanol production: a review.

  2002cited by this paper
• Regulation of Endo-Acting Glycosyl Hydrolases in the Hyperthermophilic Bacterium Thermotoga maritima Grown on Glucan- and Mannan-Based Polysaccharides

  2002cited by this paper
• Engineering proteins for thermostability: the use of sequence alignments versus rational design and directed evolution.

  2001cited by this paper
• Studies of the ankyrin repeats of the Drosophila melanogaster Notch receptor. 2. Solution stability and cooperativity of unfolding.

  2001cited by this paper
• Hyperthermophilic Enzymes: Sources, Uses, and Molecular Mechanisms for Thermostability

  2001cited by this paper
• Linear extrapolation method of analyzing solvent denaturation curves

  2000cited by this paper
• Molecular determinants of xylose isomerase thermal stability and activity: analysis of thermozymes by site-directed mutagenesis.

  2000cited by this paper
• The ankyrin repeat: a diversity of interactions on a common structural framework.

  1999cited by this paper
• Insertional gene fusion technology

  1999cited by this paper
• Chemical modification of enzymes for enhanced functionality.

  1999cited by this paper
• Mechanical and chemical unfolding of a single protein: a comparison.

  1999cited by this paper
• Dissection of the gene of the bifunctional PGK‐TIM fusion protein from the hyperthermophilic bacterium Thermotoga maritima: Design and characterization of the separate triosephosphate isomerase

  1997cited by this paper
• Creating a bifunctional protein by insertion of β-lactamase into the maltodextrin-binding protein

  1997cited by this paper
• The cellulosome: an exocellular, multiprotein complex specialized in cellulose degradation.

  1996cited by this paper
• The crystal structure of endoglucanase CelA, a family 8 glycosyl hydrolase from Clostridium thermocellum.

  1996cited by this paper
• Analysis of a Thermotoga maritima DNA fragment encoding two similar thermostable cellulases, CelA and CelB, and characterization of the recombinant enzymes.

  1996cited by this paper
• Purification of Thermotoga maritima enzymes for the degradation of cellulosic materials

  1995cited by this paper
• Denaturant m values and heat capacity changes: Relation to changes in accessible surface areas of protein unfolding

  1995cited by this paper
• Enhanced protein thermostability from site-directed mutations that decrease the entropy of unfolding.

  1990cited by this paper
• Structure and stability of thermophilic enzymes. Studies on thermolysin.

  1988cited by this paper
• Cloning and expression of two cellulase genes of Clostridium cellulolyticum in Escherichia coli.

  1988cited by this paper
• Measurement of cellulase activities

  1987influential reference
• Determination and analysis of urea and guanidine hydrochloride denaturation curves.

  1986cited by this paper
• Properties of a Clostridium thermocellum Endoglucanase Produced in Escherichia coli

  1986cited by this paper
• Characterization of a cellulose-binding, cellulase-containing complex in Clostridium thermocellum

  1983cited by this paper
• Saccharification of Complex Cellulosic Substrates by the Cellulase System from Clostridium thermocellum

  1982cited by this paper
• Determining globular protein stability: guanidine hydrochloride denaturation of myoglobin.

  1979cited by this paper
• Urea and guanidine hydrochloride denaturation of ribonuclease, lysozyme, alpha-chymotrypsin, and beta-lactoglobulin.

  1974cited by this paper
• Thermodynamics of protein denaturation. A calorimetric study of the reversible denaturation of chymotrypsinogen and conclusions regarding the accuracy of the two-state approximation.

  1970cited by this paper
• Thermodynamics of the unfolding of beta-lactoglobulin A in aqueous urea solutions between 5 and 55 degrees.

  1968cited by this paper
• Spectroscopic determination of tryptophan and tyrosine in proteins.

  1967cited by this paper
• On thermodynamics of protein denaturation

  1961cited by this paper
• Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar

  1959cited by this paper

• A guide to studying protein aggregation

  2021cites this paper
• Expression of a highly active β-glucosidase from Aspergillus niger AS3.4523 in Escherichia coli and its application in gardenia blue preparation

  2020cites this paper
• Engineering and functionalization of large circular tandem repeat protein nanoparticles

  2020cites this paper
• Construction of a trifunctional cellulase and expression in Saccharomyces cerevisiae using a fusion protein

  2018cites this paper
• Synergistic effect of the enzyme complexes comprising agarase, carrageenase and neoagarobiose hydrolase on degradation of the red algae.

  2018cites this paper
• Stabilization of Bacillus circulans xylanase by combinatorial insertional fusion to a thermophilic host protein

  2017cites this paper
• Molecular Engineering of Enzymes

  2017cites this paper
• Nanotechnology for Synthetic Biology: Crossroads Throughout Spatial Confinement

  2017cites this paper
• Nanoscale Engineering of Designer Cellulosomes

  2016cites this paper
• Synergistic enhancement of cellulase pairs linked by consensus ankyrin repeats: Determination of the roles of spacing, orientation, and enzyme identity

  2016cites this paper
• Modular and Multifunctional Enzyme Systems for Plant Cell Wall Degradation: Diversity, Synergy, Chimeras and Magnetic-Glycosidases

  2016cites this paper
• Consolidated bioprocessing for biofuel production: recent advances

  2015cites this paper
• Molecular Determinants for Protein Stabilization by Insertional Fusion to a Thermophilic Host Protein

  2015cites this paper
• Signal peptide of cellulase

  2014cites this paper
• Biomolecular assembly strategies to develop potential artificial cellulosomes

  2014cites this paper