Parishin A Inhibits Oral Squamous Cell Carcinoma via the AKT/mTOR Signaling Pathway

Background: Oral squamous cell carcinoma (OSCC) is an aggressive cancer with limited treatment options. Parishin A, a natural compound derived from Gastrodia elata, possesses multiple therapeutic properties. However, its effects on OSCC remain unexplored. Purpose: This study explores the anti-cancer potential of Parishin A on OSCC and its mechanisms. Methods: OSCC cell lines YD-10B and Ca9-22 were treated with varying Parishin A concentrations. Cell viability was detected using the CCK-8 assay, and colony formation was evaluated in agarose gel. Migration and invasion ability were assessed through wound healing and Matrigel invasion assays. The protein expression levels involved in the PI3K/AKT/mTOR signaling pathway and epithelial–mesenchymal transition (EMT) markers were examined via Western blotting. Results: Parishin A inhibited OSCC cell viability in both dose- and time-dependent manners, with significant reductions at 20, 40, 60, and 80 μM, without affecting normal human gingival fibroblasts. Colony formation decreased substantially at ≥40 μM higher Parishin A concentrations in a dose-dependent manner. Also, migration and invasion assays showed significant suppression by Parishin A treatment concentration ≥40 μM in a dose-dependent manner, as evidenced by decreased wound closure and invasion. Western blot analyses revealed increased E-cadherin levels and decreased N-cadherin and vimentin levels, suggesting EMT inhibition. Parishin A also decreased the phosphorylation levels of PI3K, AKT, and mTOR. Conclusion: Collectively, these findings support the potential of Parishin A as an anti-OSCC agent.

• Publication year

  2024

• Venue

  Pharmaceuticals

• Publication date

  2024-09-26

• Fields of study

  Medicine

• Identifiers
  DOI 10.3390/ph17101277PMID 39458918PMCID 11510427
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Imatinib inhibits oral squamous cell carcinoma by suppressing the PI3K/AKT/mTOR signaling pathway

  2024cited by this paper
• Integrating natural compounds and nanoparticle‐based drug delivery systems: A novel strategy for enhanced efficacy and selectivity in cancer therapy

  2024cited by this paper
• PI3K/AKT/mTOR signaling transduction pathway and targeted therapies in cancer

  2023cited by this paper
• Dioscin inhibiting EGFR-mediated Survivin expression promotes apoptosis in oral squamous cell carcinoma cells

  2023cited by this paper
• Epidemiology, Risk Factors, and Prevention of Head and Neck Squamous Cell Carcinoma

  2023cited by this paper
• Rhein Induces Oral Cancer Cell Apoptosis and ROS via Suppresse AKT/mTOR Signaling Pathway In Vitro and In Vivo

  2023cited by this paper
• Parishin alleviates vascular ageing in mice by upregulation of Klotho

  2023cited by this paper
• Parishin A-loaded mesoporous silica nanoparticles modulate macrophage polarization to attenuate tendinopathy

  2023cited by this paper
• Oral Squamous Cell Carcinoma and Concomitant Primary Tumors, What Do We Know? A Review of the Literature

  2023cited by this paper
• Oral squamous cell carcinomas: state of the field and emerging directions

  2023cited by this paper
• Parishin From Gastrodia Elata Ameliorates Aging Phenotype in Mice in a Gut Microbiota-Related Manner

  2022cited by this paper
• Gastrodiae rhizoma attenuates brain aging via promoting neuritogenesis and neurodifferentiation.

  2021cited by this paper
• Polysaccharides like pentagalloylglucose, parishin a and stevioside inhibits the viral entry by binding the Zika virus envelope protein

  2020cited by this paper
• Squamous cell carcinoma of the oral cavity, larynx, oropharynx and hypopharynx: EHNS-ESMO-ESTRO Clinical Practice Guidelines for diagnosis, treatment and follow-up.

  2020cited by this paper
• EMT, cancer stem cells and autophagy; The three main axes of metastasis.

  2020cited by this paper
• Role of AKT and mTOR Signaling Pathways in the Induction of Epithelial-Mesenchymal Transition (EMT) Process.

  2019cited by this paper
• EMT Transition States during Tumor Progression and Metastasis.

  2019cited by this paper
• Targeted disruption of PI3K/Akt/mTOR signaling pathway, via PI3K inhibitors, promotes growth inhibitory effects in oral cancer cells

  2018cited by this paper
• Targeting the PI3K/AKT/mTOR signaling pathway as an effectively radiosensitizing strategy for treating human oral squamous cell carcinoma in vitro and in vivo

  2017cited by this paper
• Explant culture: An advantageous method for isolation of mesenchymal stem cells from human tissues

  2017cited by this paper
• Gastrodia elata and epilepsy: Rationale and therapeutic potential.

  2016cited by this paper
• Cancer of the oral cavity.

  2015cited by this paper
• Targeting PI3K/Akt/mTOR Signaling in Cancer

  2014cited by this paper
• Gastrodin stimulates anticancer immune response and represses transplanted H22 hepatic ascitic tumor cell growth: Involvement of NF-κB signaling activation in CD4+ T cells.

  2013cited by this paper
• AKT/PKB signaling: navigating downstream.

  2007cited by this paper
• Exploiting the PI3K/AKT Pathway for Cancer Drug Discovery

  2005cited by this paper
• Clinical, cellular, and molecular aspects of cancer invasion.

  2003cited by this paper
• Effects of naturally occurring glucosides, solasodine glucosides, ginsenosides and parishin derivatives on multidrug resistance of lymphoma cells and leukocyte functions.

  2001cited by this paper
• Proliferation, cell cycle and apoptosis in cancer

  2001cited by this paper

• Sestrin2 Knockdown Impairs Proliferation, Migration, Invasion, and Apoptosis in OSCC Cells via PI3K/AKT/mTOR and MAPK Pathways

  2025cites this paper
• Simultaneous Quantification of Multiple Analytes in Rat Plasma by UHPLC–MS/MS Following Oral Administration of Gastrodiae Rhizoma Extract for Pharmacokinetic Evaluation

  2025cites this paper
• LAMP1 enhances DNA-PKcs-mediated AKT phosphorylation and VEGFC secretion to promote lymph node metastasis in esophageal squamous cell carcinoma

  2025cites this paper
• Screening the grading markers and their application in the grade discrimination of Gastrodiae Rhizoma using metabolomics and machine learning

  2025cites this paper
• Isoquercitrin Suppresses Esophageal Squamous Cell Carcinoma (ESCC) by Inducing Excessive Autophagy and Promoting Apoptosis via the AKT/mTOR Signaling Pathway

  2025cites this paper
• Dysregulated PI3K/AKT signaling in oral squamous cell carcinoma: The tumor microenvironment and epigenetic modifiers as key drivers

  2025cites this paper
• Innovative dual-target AKT inhibitors unveiling novel mechanisms and translational potential.

  2025cites this paper
• MK2-mediated AKT/MYC signaling activation promotes epithelial-mesenchymal transition in lung adenocarcinoma

  2025cites this paper
• Advances in Preventive and Therapeutic Strategies for Oral Cancer: A Short Review

  2024cites this paper
• Evolving Therapeutic Strategies in Esophageal Squamous Cell Carcinoma: Advances and Perspectives

  2024cites this paper