Comparison between Inhibition of CatSper and KSper Channels with NNC 55-0396 and Quinidine on Human Sperm Function

Background: Calcium enters human sperm through the “Cation Channel of Sperm” (CatSper), while potassium ions exit via the sperm potassium channel (KSper). These two channels regulate intracellular calcium concentration ([Ca2+]i) and membrane potential. Our study aims to investigate and compare the contributions of these channels in capacitated sperm function. Methods: Results: NNC and quinidine significantly decreased progressive sperm motility (P=0.001) and reduced sperm kinematics (P=0.001). NNC but not quinidine significantly decreased sperm survival (P=0.001), reduced [Ca2+]i in live spermatozoa (P=0.05), and induced the acrosomal reaction (P=0.012). Conclusion: Inhibition of KSper without effect on [Ca2+]i can inhibit sperm motility and increase mortality rate. It seems that the function of KSper is as vital as CatSper in human sperm physiology.

• Publication year

  2025

• Venue

  Iranian Journal of Medical Sciences

• Publication date

  2025-03-01

• Fields of study

  Medicine

• Identifiers
  DOI 10.30476/ijms.2024.102383.3528PMID 40224202PMCID 11992345
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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