High-intensity exercise alongside insulin alleviates muscle atrophy in type 1 diabetes mellitus concomitant with modulation of mitophagy-related proteins in skeletal muscle

Abstract Background Diabetes patients’ quality of life can be severely impacted by diabetic muscle atrophy. Aim This study aimed to explore the impact of high-intensity exercise (HIE) alongside insulin treatment on muscle atrophy in a rat model of type 1 diabetes mellitus (T1DM). Methodology Fifty rats were allocated into five groups; Group 1, control sedentary (CS), T1DM was elicited in the rest of the groups by giving them Streptozotocin (STZ) (60 mg/kg), where group 2 (DS) remained sedentary, while groups 3,4,5 were treated with insulin after induction of diabetes. Group 4 (DI+MIE) and 5 (DI+ HIE) underwent moderate and high-intensity exercise, respectively. Results HIE for 14 days combined with insulin treatment significantly restored muscle strength and mass with a significant modification in the mitophagy-related proteins and fibroblast growth factor 21 (FGF 21) compared to other treated groups. Conclusion This study concluded that there is a therapeutic role for HIE with insulin against T1DM-induced muscle atrophy. KEY POINTS T1DM induces loss of skeletal muscle mass and strength. T1DM enhances muscle atrophy-related genes (MAFbx and MuRF1) and impairs mitochondrial function. HIE alongside insulin treatment restores muscle mass, strength, and histological architecture in T1DM-induced muscle atrophy model. HIE alongside insulin treatment over MIE moderated the mitochondrial dysfunction via modulation of the mitophagy-related mediators (BNIP3, Parkin, P62, and LC3II/LC3I) and FGF 21 expression.

• Publication year

  2024

• Venue

  Archives of Physiology and Biochemistry

• Publication date

  2024-10-09

• Fields of study

  Medicine

• Identifiers
  DOI 10.1080/13813455.2024.2410791PMID 39382178
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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