Chemical Synthesis of Site-Specifically 2′-Azido-Modified RNA and Potential Applications for Bioconjugation and RNA Interference

One of the most important scientific discoveries in the recent past concerns RNA interference (RNAi), which is a post-transcriptional gene-silencing mechanism induced by small interfering RNA (siRNA) and micro-RNA (miRNA).[1] RNAi has opened up new avenues in the development of siRNA and miRNA as therapeutic agents for various diseases.[2] The reason for the large number of reports about chemically modified siRNA is their potential to enhance nuclease resistance, to prevent immune activation, to decrease off-target effects, and to improve pharmacokinetic and pharmacodynamic properties, all of which are important for the application of siRNA as therapeutic agents.[3] Another substantial challenge is siRNA delivery, because these reagents cannot easily traverse cell membranes because of their size and negative charge.[4] To date, the most promising therapeutic approach based on RNAi involves chemically modified siRNA that can resolve some of the issues mentioned above.

• Publication year

  2010

• Venue

  ChemBioChem

• Publication date

  2010-12-17

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1002/cbic.201000646PMID 21171007PMCID 3193913
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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