The effect of flue-curing and redrying on the diversity of fungal communities in tobacco leaves

Flue-curing and redrying are important processing stages before tobacco fermentation, closely linked to microbial actions that influence the fermentation process. It is necessary to investigate the effects of flue-curing and redrying on diversity and succession of tobacco fungal communities. It was shown that a total of 9 phyla, 33 classes, 94 orders, 266 families, 646 genera, and 6,396 amplicon sequence variants (ASVs) were identified in the fungi communities of 36 samples from different processing stages (before flue-curing, after flue-curing, before redrying and after redrying) based on high-throughput sequencing technology. Dominant genera shared by tobacco leaves at different stages were Alternaria and Sampaiozyma. About 80% of fungi in stored tobacco leaves after redrying originated from fresh tobacco leaves before flue-curing, while the rest were primarily enriched in the post-harvest processing environment. After flue-curing, major molds like Aspergillus and Penicillium were notably enriched. The distribution of fungal communities suggested that the flue-curing and redrying had a significant impact on fungal composition. Functional annotation of fungal communities at the guild level exhibited differences during processing stages. Main fungal functional groups were identified. In summary, our study elucidated dynamic changes in the composition of fungal communities and highlighted key stages in mold enrichment during tobacco leaf processing, laying groundwork for mildew prevention and control during tobacco leaf fermentation.

• Publication year

  2024

• Venue

  BMC Microbiology

• Publication date

  2024-11-23

• Fields of study

  Biology, Agricultural and Food Sciences, Medicine, Environmental Science

• Identifiers
  DOI 10.1186/s12866-024-03635-4PMID 39580418PMCID 11585231
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Characterization of bacterial community in tobacco leaves at flue-curing and redrying processing stages

  2023cited by this paper
• Widely targeted metabolomics analysis reveals the mechanism of quality improvement of flue-cured tobacco

  2022cited by this paper
• The effect of flue-curing procedure on the dynamic change of microbial diversity of tobaccos

  2021cited by this paper
• Fungal Composition and Diversity of the Tobacco Leaf Phyllosphere During Curing of Leaves

  2020cited by this paper
• Spoilage fungi in a bread factory in Brazil: Diversity and incidence through the bread-making process.

  2019cited by this paper
• Microfluidics and Metabolomics Reveal Symbiotic Bacterial–Fungal Interactions Between Mortierella elongata and Burkholderia Include Metabolite Exchange

  2019cited by this paper
• The effects of threshing and redrying on bacterial communities that inhabit the surface of tobacco leaves

  2017cited by this paper
• DADA2: High resolution sample inference from Illumina amplicon data

  2016cited by this paper
• Dispersal in microbes: fungi in indoor air are dominated by outdoor air and show dispersal limitation at short distances

  2013cited by this paper
• Fungal growth pattern, sources and factors of mould contamination in a dry-cured meat production facility.

  2010cited by this paper
• Preparation of Genomic DNA from Bacteria

  2001cited by this paper
• Fungi Isolated from Flue-cured Tobacco at Time of Sale and After Storage

  1969cited by this paper

• Molecular and Fluorometric based approach for the detection and isolation of aflatoxin producing Aspergillus sp. in chewing or smokeless tobacco.

  2025cites this paper
• 5'UTR Dynamics: Modulating Model Plant Defense Mechanisms against Fungal Threats.

  2025cites this paper
• Enhancing tobacco quality and flavor through cold plasma treatment: structural, chemical, and sensory modifications

  2025cites this paper