Engineering protein folding and translocation improves heterologous protein secretion in Saccharomyces cerevisiae

No abstract is available for this paper.

• Publication year

  2015

• Venue

  Biotechnology and Bioengineering

• Publication date

  2015-09-01

• Fields of study

  Biology, Medicine, Engineering

• Identifiers
  DOI 10.1002/bit.25596PMID 25850421
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  2006influential reference
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  2006cited by this paper
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  2004cited by this paper
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  1998influential reference
• Signal sequence recognition in posttranslational protein transport across the yeast ER membrane.

  1998cited by this paper
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  1997cited by this paper
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  1996cited by this paper
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  1996cited by this paper
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  1996cited by this paper
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  1996cited by this paper
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  1995cited by this paper
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  1995cited by this paper
• Signal sequence recognition and protein targeting to the endoplasmic reticulum membrane.

  1994cited by this paper
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  1994cited by this paper
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  1994cited by this paper
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  1994cited by this paper
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  1992cited by this paper
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  1992cited by this paper
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  1992cited by this paper
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  1987cited by this paper
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  1974cited by this paper
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  1974cited by this paper
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  1973cited by this paper

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