The B-type cyclin Clb4 prevents meiosis I sister centromere separation in budding yeast

In meiosis, one round of DNA replication followed by two rounds of chromosome segregation halves the ploidy of the original cell. Accurate chromosome segregation in meiosis I depends on recombination between homologous chromosomes. Sister centromeres attach to the same spindle pole in this division and only segregate in meiosis II. We used budding yeast to select for mutations that produced viable spores in the absence of recombination. The most frequent mutations inactivated CLB4, which encodes one of four B-type cyclins. In two wild yeast isolates, Y55 and SK1, but not the W303 laboratory strain, deleting CLB4 causes premature sister centromere separation and segregation in meiosis I and frequent termination of meiosis after a single division, demonstrating a novel role for Clb4 in meiotic chromosome dynamics and meiotic progression. This role depends on the genetic background since meiosis in W303 is largely independent of CLB4.

• Publication year

  2024

• Venue

  bioRxiv

• Publication date

  2024-12-19

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1101/2024.12.18.629243PMID 39763826PMCID 11702657
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• CDK Regulation of Meiosis: Lessons from S. cerevisiae and S. pombe

  2020cited by this paper
• Reductional Meiosis I Chromosome Segregation Is Established by Coordination of Key Meiotic Kinases

  2019cited by this paper
• Inexpensive Multiplexed Library Preparation for Megabase-Sized Genomes

  2015cited by this paper
• Improved use of a public good selects for the evolution of undifferentiated multicellularity

  2013cited by this paper
• Meiosis I chromosome segregation is established through regulation of microtubule–kinetochore interactions

  2012influential reference
• Meiosis I is established through division-specific translational control of a cyclin.

  2008cited by this paper
• Population genomics of domestic and wild yeasts

  2008cited by this paper
• Shugoshin promotes sister kinetochore biorientation in Saccharomyces cerevisiae.

  2007cited by this paper
• Kinetochore orientation during meiosis is controlled by Aurora B and the monopolin complex.

  2007cited by this paper
• Monopolar attachment of sister kinetochores at meiosis I requires casein kinase 1.

  2006cited by this paper
• Sister-chromatid cohesion mediated by the alternative RF-CCtf18/Dcc1/Ctf8, the helicase Chl1 and the polymerase-α-associated protein Ctf4 is essential for chromatid disjunction during meiosis II

  2004cited by this paper
• Recycling the Cell Cycle Cyclins Revisited

  2004cited by this paper
• Selective killing of vegetative cells in sporulated yeast cultures by exposure to diethyl ether

  2004cited by this paper
• Recycling the cell cycle: cyclins revisited.

  2004cited by this paper
• Kinetochore recruitment of two nucleolar proteins is required for homolog segregation in meiosis I.

  2003cited by this paper
• Spo13 protects meiotic cohesin at centromeres in meiosis I.

  2002cited by this paper
• Requirement of the spindle checkpoint for proper chromosome segregation in budding yeast meiosis.

  2000cited by this paper
• Functional genomics identifies monopolin: a kinetochore protein required for segregation of homologs during meiosis i.

  2000cited by this paper
• Slk19p is necessary to prevent separation of sister chromatids in meiosis I.

  2000cited by this paper
• The spindle checkpoint of budding yeast depends on a tight complex between the Mad1 and Mad2 proteins.

  1999cited by this paper
• Regulation of Cdc28 Cyclin-Dependent Protein Kinase Activity during the Cell Cycle of the Yeast Saccharomyces cerevisiae

  1998cited by this paper
• An atypical topoisomerase II from archaea with implications for meiotic recombination

  1997cited by this paper
• Cohesins: chromosomal proteins that prevent premature separation of sister chromatids.

  1997cited by this paper
• Meiosis-specific DNA double-strand breaks are catalyzed by Spo11, a member of a widely conserved protein family.

  1997cited by this paper
• GFP tagging of budding yeast chromosomes reveals that protein-protein interactions can mediate sister chromatid cohesion.

  1996cited by this paper
• Spc42p: a phosphorylated component of the S. cerevisiae spindle pole body (SPD) with an essential function during SPB duplication

  1996cited by this paper
• Specialization of B-type cyclins for mitosis or meiosis in S. cerevisiae.

  1995cited by this paper
• The yeast MER2 gene is required for chromosome synapsis and the initiation of meiotic recombination.

  1995cited by this paper
• Homologous pairing is reduced but not abolished in asynaptic mutants of yeast

  1994cited by this paper
• MEI4, a meiosis-specific yeast gene required for chromosome synapsis

  1992cited by this paper
• Characterization of four B-type cyclin genes of the budding yeast Saccharomyces cerevisiae.

  1992cited by this paper
• Feedback control of mitosis in budding yeast.

  1991cited by this paper
• Analysis of wild-type and rad50 mutants of yeast suggests an intimate relationship between meiotic chromosome synapsis and recombination.

  1990cited by this paper
• G1-specific cyclins of S. cerevisiae: cell cycle periodicity, regulation by mating pheromone, and association with the p34CDC28 protein kinase.

  1990cited by this paper
• Genetic control of chromosome synapsis in yeast meiosis.

  1989cited by this paper
• The role of the SPO11 gene in meiotic recombination in yeast.

  1985influential reference
• One-step gene disruption in yeast.

  1983cited by this paper
• Meiosis in haploid yeast.

  1982cited by this paper

• The B-type cyclin Clb4 prevents meiosis I sister centromere separation in budding yeast

  2025cites this paper