High-protein diet based on whey protein isolate preserves muscle mass and its association with resistance training attenuates glomerular hypertrophy in rats.

OBJECTIVE To evaluate the effect of administering a high-protein diet (HP) based on whey protein isolate (WPI) associated with ST in rats. METHODS Twenty-two Wistar rats were fed a diet containing either 14% WPI (normoproteic) or 35% WPI (high protein) and were subjected to stair training or kept sedentary for 12 weeks. SN = sedentary normoproteic; SH = sedentary high protein; TN = trained normoproteic; TH = trained high protein. RESULTS HP decreases food intake (P < 0.0001). The SH group showed a significant reduction in plasma triglycerides (P = 0.03). Quadriceps weight was greatest in TH, followed by SH and TN (P < 0.0001). Kidney weight was greater in TH, followed by SH and TN, and was greater than in SN (P < 0.0001). Urea levels were lowest in the SN group (P < 0.001). The urinary space was larger in the TH and SH groups. HP and ST increased the cross-sectional area (CSA) of the gastrocnemius (P < 0.0001) and quadriceps (P < 0.0001) muscles. Hepatic glycogen deposits were highest in the SH group (P < 0.0001). CONCLUSIONS HP with 35% whey promoted satiety and increased hepatic glycogen content without affecting glycemia. Its combination with ST was more efficient in increasing muscle hypertrophy, altering plasma urea levels, and enlarging the urinary space. These findings may be related to the adaptive process of renal physiology stimulated by HP.

• Publication year

  2025

• Venue

  Nutrition (Burbank, Los Angeles County, Calif.)

• Publication date

  2025-02-01

• Fields of study

  Medicine

• Identifiers
  DOI 10.1016/j.nut.2025.112708PMID 40010167
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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