Engineering UDP‐sugar synthesis pathways for exopolysaccharide biosynthesis in Streptococcus thermophilus AR333

Exopolysaccharide (EPS) produced by Streptococcus thermophilus can significantly improve the viscosity, texture and taste of dairy products, which have broad application potential in the food industry. However, EPS production is typically low (< 100 mg L−1) in S. thermophilus, making it difficult to meet industrialization requirements. The utilization of metabolic engineering for genetic modification of S. thermophilus is an efficient approach to enhance EPS biosynthesis.

• Publication year

  2025

• Venue

  The Journal of the Science of Food and Agriculture

• Publication date

  2025-03-03

• Fields of study

  Medicine, Engineering

• Identifiers
  DOI 10.1002/jsfa.14206PMID 40028768
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  2017influential reference
• Characterization of a yogurt-quality improving exopolysaccharide from Streptococcus thermophilus AR333

  2017cited by this paper
• Microbial production of lactic acid: the latest development.

  2016cited by this paper
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  2016cited by this paper
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  2016influential reference
• EpsA is an essential gene in exopolysaccharide production in L actobacillus johnsonii FI9785

  2015cited by this paper
• Shuttle expression plasmids for genetic studies in Streptococcus mutans.

  2008cited by this paper
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  2007cited by this paper
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  2000cited by this paper
• Identification and characterization of the eps (Exopolysaccharide) gene cluster from Streptococcus thermophilus Sfi6

  1996cited by this paper
• Lactose transport system of Streptococcus thermophilus. The role of histidine residues.

  1992cited by this paper
• Lactose Transport System of Streptococcus thermophilus

  1992cited by this paper

• Streptococcus thermophilus: A next-generation chassis for customizing exopolysaccharide with rare sugar.

  2025cites this paper