Impact of mixed carbon sources on microbial function and network in hypersaline denitrification.

This study evaluated denitrification systems using two carbon sources: glucose alone (G), and a mixture of glucose, methanol, and sodium acetate (GMS), treating synthetic saline wastewater to prove the superior operational efficiency of the mixed carbon sources. Both systems showed excellent nitrogen removal under 80 g-NaCl L-1, but the glucose-based system exhibited unstable nitrate removal at a low C/N ratio of 3. Microbial community composition was distinct between the systems the GMS system showed highest organic removal due to the abundance of Marinobacter spp., and the prediction of functional genes using phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) showed an increase of denitrifying genes such as nitrate (Nar), and nitrite (Nir) reductase in GMS. Co-occurrence network analysis revealed that GMS guaranteed diverse microbial interactions and stable network structure, facilitating enhanced denitrification capability. This study offers engineering insights into microbial denitrification under hypersaline conditions.

• Publication year

  2025

• Venue

  Bioresource Technology

• Publication date

  2025-03-01

• Fields of study

  Medicine, Environmental Science

• Identifiers
  DOI 10.1016/j.biortech.2025.132366PMID 40056958
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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