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Method for Selecting the Down‐Sampling Factor of Photoacoustic Image by Using Cumulative Power Difference in Frequency Domain

Shihao Tang,Min Wan,Yameng Zhang,Jiani Li,Ling Tao,Weitao Li

Published 2025 in Journal of Biophotonics

ABSTRACT

As a novel non‐invasive imaging technology, a constraint of photoacoustic microscopy (PAM) is its imaging speed. Often, PAM utilizes sparse spatial sampling, which necessitates extensive prior experimentation to accurately select the down‐sampling factors. To overcome this limitation, this study proposes a frequency‐domain evaluation index, cumulative power difference (CPD), for rapid selection of the optimal down‐sampling factor. We apply the proposed CPD to photoacoustic images of the ear and brain of the mouse. The result shows that as the down‐sampling factor increases, there is a similar decreasing trend in the quality of the 20 images. CPD was significantly correlated with PCC/MSE/SSIM (p < 0.001). The findings suggest that CPD, with its ability to evaluate the quality of photoacoustic images and quickly quantify the quality loss of down‐sampled images without prior inspection. This study contributes to expanding the application range of PAM and supporting its clinical prospects.

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