Transcriptome Response of Rosa roxburghii Fruit to Top-Rot Disease and Functional Analysis of RrHSP18.1 in Top-Rot Disease Resistance.

Top-rot disease (TRD) in Rosa roxburghii fruit is caused by Colletotrichum fructicola. TRD has emerged as a significant concern due to its frequent occurrence and cause of substantial economic losses. To understand the transcriptome response to TRD infection and identify candidate genes involved in TRD resistance, four critical time points (0, 24, 72, and 120 hpi) fruit tissues inoculated with TRD pathogen were selected for RNA-seq. A total of 1,890 differentially expressed genes (DEGs) were identified from the transcriptome data, including 1,051 upregulated and 839 downregulated genes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed that they were primarily involved in oxidoreductase activity and the synthesis and metabolism secondary metabolites. Many putative transcription factor families such as MYB, bHLH, AP2-EREBP, NAC, and WRKY were also identified as being positively responsive to TRD infestation. Among the upregulated genes, RrHSP18.1 exhibited the strongest response to TRD. Transient overexpression and gene silencing demonstrated that RrHSP18.1 positively regulated TRD resistance in R. roxburghii fruit, partially through promoting the expression of antioxidant-related genes and enhancing their enzyme activities. Collectively, the results facilitated a better understanding of the transcriptional response to TRD and offered candidate genes for developing R. roxburghii germplasm resource with improved TRD resistance.

• Publication year

  2025

• Venue

  Phytopathology

• Publication date

  2025-03-31

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1094/PHYTO-01-25-0015-RPMID 40163756
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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