The Analysis of the Glycosyltransferase Activity Gene Family in Gossypium hirsutum and Functional Verification of GTs Conferring Resistance to Verticillium Wilt

Glycosyltransferases (GTs) play an important role in plant growth and development, as well as responses to biotic and abiotic stresses. However, the function of the GT family in cotton resistance to Verticillium wilt is limited. In the present study, transcriptome analysis revealed eight GTs upregulated in susceptible cotton varieties and downregulated in resistant cotton varieties during early Verticillium dahliae inoculation, indicating they were involved in regulating the infection of V. dahliae in cotton. Promoter analysis revealed a high prevalence of MeJA (methyl jasmonate) and ABA (abscisic acid)-related cis-acting elements among these GTs. Genome-wide and location analysis of the homologous genes showed that these GTs were relatively conserved in evolution. Furthermore, a Virus-Induced Gene Silencing (VIGS) experimental results demonstrated a reduction in disease resistance after GhGT61 silencing. These insights not only deepen our understanding of the GT family’s role in cotton, but also provide a foundation for future research on the disease resistance mechanisms of these genes.

• Publication year

  2025

• Venue

  International Journal of Molecular Sciences

• Publication date

  2025-03-29

• Fields of study

  Biology, Agricultural and Food Sciences, Medicine, Environmental Science

• Identifiers
  DOI 10.3390/ijms26073170PMID 40243945PMCID 11989804
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Two pathogen-inducible UDP-glycosyltransferases, UGT73C3 and UGT73C4, catalyze the glycosylation of pinoresinol to promote plant immunity in Arabidopsis

  2025cited by this paper
• Combined Transcriptome and Metabolome Analysis Reveals That Carbon Catabolite Repression Governs Growth and Pathogenicity in Verticillium dahliae

  2024cited by this paper
• MAPK and phenylpropanoid metabolism pathways involved in regulating the resistance of upland cotton plants to Verticillium dahliae

  2024cited by this paper
• Overexpression of a GIPC glycosyltransferase gene, OsGMT1, suppresses plant immunity and delays heading time in rice.

  2023cited by this paper
• Genome-wide analysis of serine carboxypeptidase-like protein (SCPL) family and functional validation of Gh_SCPL42 unchromosome conferring cotton Verticillium der Verticillium wilt stress in Gossypium hirsutum

  2022cited by this paper
• OsTGAL1 suppresses the resistance of rice to bacterial blight disease by regulating the expression of salicylic acid glucosyltransferase OsSGT1.

  2022cited by this paper
• UGT76B1, a promiscuous hub of small molecule-based immune signaling, glucosylates N-hydroxypipecolic acid, and balances plant immunity.

  2021cited by this paper
• Genome-wide identification of the DUF668 gene family in cotton and expression profiling analysis of GhDUF668 in Gossypium hirsutum under adverse stress

  2021cited by this paper
• Salicylic acid carboxyl glucosyltransferase UGT87E7 regulates disease resistance in Camellia sinensis

  2021cited by this paper
• Genome-wide analysis of the serine carboxypeptidase-like protein family in Triticum aestivum reveals TaSCPL184-6D is involved in abiotic stress response

  2021cited by this paper
• Genome-wide identification of the DUF668 gene family in cotton and expression profiling analysis of GhDUF668 in Gossypium hirsutum under adverse stress

  2021cited by this paper
• UGT76B1, a promiscuous hub of small molecule-based immune signaling, glucosylates N-hydroxypipecolic acid, and balances plant immunity

  2021cited by this paper
• A novel pathogen-responsive glycosyltransferase UGT73C7 mediates the redirection of phenylpropanoid metabolism and promotes SNC1-dependent Arabidopsis immunity.

  2021cited by this paper
• OUP accepted manuscript

  2021cited by this paper
• Transcriptomic analysis of gene expression of Verticillium dahliae upon treatment of the cotton root exudates

  2020cited by this paper
• TaUGT6, a Novel UDP-Glycosyltransferase Gene Enhances the Resistance to FHB and DON Accumulation in Wheat

  2020cited by this paper
• Gossypium barbadense and Gossypium hirsutum genomes provide insights into the origin and evolution of allotetraploid cotton

  2019cited by this paper
• fastp: an ultra-fast all-in-one FASTQ preprocessor

  2018influential reference
• Transfer of tomato immune receptor Ve1 confers Ave1‐dependent Verticillium resistance in tobacco and cotton

  2017cited by this paper
• Genome-wide identification of the TIFY gene family in three cultivated Gossypium species and the expression of JAZ genes

  2017cited by this paper
• Potential of Endophytic Fungi Isolated from Cotton Roots for Biological Control against Verticillium Wilt Disease

  2017cited by this paper
• Transcription Factors Involved in Plant Resistance to Pathogens.

  2017cited by this paper
• Genome-wide analysis of UDP-glycosyltransferase super family in Brassica rapa and Brassica oleracea reveals its evolutionary history and functional characterization

  2017cited by this paper
• MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets.

  2016cited by this paper
• A Brachypodium UDP-Glycosyltransferase Confers Root Tolerance to Deoxynivalenol and Resistance to Fusarium Infection1

  2016cited by this paper
• Cotton polyamine oxidase is required for spermine and camalexin signalling in the defence response to Verticillium dahliae.

  2015cited by this paper
• Sequencing of allotetraploid cotton (Gossypium hirsutum L. acc. TM-1) provides a resource for fiber improvement

  2015cited by this paper
• Cotton WRKY1 Mediates the Plant Defense-to-Development Transition during Infection of Cotton by Verticillium dahliae by Activating JASMONATE ZIM-DOMAIN1 Expression1[C][W]

  2014cited by this paper
• Genome and Transcriptome Analysis of the Fungal Pathogen Fusarium oxysporum f. sp. cubense Causing Banana Vascular Wilt Disease

  2014cited by this paper
• Interactions between Verticillium dahliae and its host: vegetative growth, pathogenicity, plant immunity

  2014cited by this paper
• A Transcript Profiling Approach Reveals an Abscisic Acid-Specific Glycosyltransferase (UGT73C14) Induced in Developing Fiber of Ligon lintless-2 Mutant of Cotton (Gossypium hirsutum L.)

  2013cited by this paper
• Cloning and characterization of a Verticillium wilt resistance gene from Gossypium barbadense and functional analysis in Arabidopsis thaliana

  2011cited by this paper
• A Glutamic Acid-Rich Protein Identified in Verticillium dahliae from an Insertional Mutagenesis Affects Microsclerotial Formation and Pathogenicity

  2010cited by this paper
• Glycosyltransferases: structures, functions, and mechanisms.

  2008cited by this paper
• Mapping and quantifying mammalian transcriptomes by RNA-Seq

  2008cited by this paper
• Physiology and molecular aspects of Verticillium wilt diseases caused by V. dahliae and V. albo-atrum.

  2006cited by this paper
• Plant secondary metabolism glycosyltransferases: the emerging functional analysis.

  2005cited by this paper
• On the origin of family 1 plant glycosyltransferases.

  2003cited by this paper
• Rate variation among nuclear genes and the age of polyploidy in Gossypium.

  2003cited by this paper
• PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences

  2002influential reference
• Glycoside hydrolases and glycosyltransferases. Families, modules, and implications for genomics.

  2000cited by this paper

• Cold plasma-induced transcriptomic reprogramming and alternative splicing in tomato plants infected with ToBRFV

  2026cites this paper