2,4-dinitrophenyl-hapten specific hemolytic plaque-in-gel formation by mouse myeloma (MOPC-315) cells.

A mouse myeloma (MOPC-315) producing immunoglobulin A, which specifically binds 2,4-dinitrophenyl-hapten, has been shown to form hapten-specific plaques by a hemolytic plaque-in-gel assay. Sheep erythrocytes coupled with dinitrophenylated bovine serum albumin and rabbit antibody directed to the heavy chain as enhancing serum were used. Virtually all of the tumor cells formed plaques. DNP-lysine sharply inhibited plaque formation at a concentration slightly in excess of the reciprocal of the binding constant of the myeloma protein.

• Publication year

  1970

• Venue

  Journal of Immunology

• Publication date

  1970-01-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.4049/jimmunol.104.1.251PMID 4904002
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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