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SIMULTANEOUS VOLTAMMETRIC DETERMINATION OF 3-METHYLADENINE AND ADENINE AT DISPOSABLE SCREEN-PRINTED CARBON ELECTRODE.

José G. S. Neto,Wellington E. Rodrigues,Éverton Marcelo P. Diniz,V. B. Dos Santos,Severino Carlos B. Oliveira

Published 2025 in Analytical Biochemistry

ABSTRACT

The anodic behavior of 3-methyladenine (3-mAde) and adenine (Ade) were investigated in aqueous media on commercial screen-printed carbon electrodes (SPCEs), using cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The electrochemical performance of the SPCEs was first evaluated by CV and using the [Fe(CN)6]3- redox probe. The electroactive areas of some SPCEs were also determined and discussed. Overall, the results were very satisfactory and indicated good repeatability and reproducibility of the SPCEs. Regarding 3-mAde its oxidation occurred in a single irreversible step pH dependent controlled by a diffusion layer and at more positive potential, ∼ +200.00 mV, in relation to Ade, demonstrating the possibility of simultaneous detection of these two biomarkers. By DPV experimental conditions were explored to increase the sensitivity and selectivity of the 3-mAde electrooxidation signal, such as influence of the composition and pH of the medium, effect of 3-mAde concentration and presence of possible interferents. A simple, sensitive, selective and fast electroanalytical method, using DPV and commercial SPCE, was developed for determination of 3-mAde in hydrolyzed DNA samples; with a linear range from 2.00 to 10.00 μmol L-1, limit of detection (LOD) of 0.35 μmol L-1 and recoveries ranged from 94.0% to 102% in acetate buffer (pH = 4.10). For the simultaneous quantification of Ade and 3-mAde the analytical data were: concentration range of 0.50 - 10.00 μmol L-1, LOD of 0.34 μmol L-1 and the mean recovery was 101% for Ade and from 2.00 to 10.00 μmol L-1, LOD of 0.71 μmol L-1 and the mean recovery was 95.2% for 3-mAde.

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