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Systematic expression analysis of pecan GATA gene family during graft healing reveals that CiGATA8b and CiGATA12a are involved in stress responses.

Yan Zhang,J. Xuan,Jilin Zuo,Mengxin Hou,Guoming Wang,Min Zhai,Longjiao Hu,Xiaofang Ouyang,Z. Mo

Published 2025 in International Journal of Biological Macromolecules

ABSTRACT

GATA transcription factors are type IV zinc-finger proteins that could bind to the GATA motif within the promoters of downstream genes, thus influencing plant development and stress responses. Presently, pecan GATA (CiGATA) gene family has yet to be systematically characterized. In this study, 33 CiGATA members were identified and grouped into four classes, with genes within the same class exhibiting structural commonality. Cis-Elements in the promoters of CiGATAs were predicted to be mainly associated with light, abscisic acid, methyl jasmonate, and anaerobic induction. Four members including CiGATA8b/12a/1b/3b were highly responsive to graft healing, among which CiGATA8b and CiGATA12a were likely related to the stress responses during graft healing, as revealed by the annotation of their co-expressed genes. CiGATA8b and CiGATA12a were both located in the nucleus and acted as transcriptional suppressor and activator, respectively. Yeast one-hybrid indicated that CiGATA8b and CiGATA12a could bind to the promoters of CiNLR and CiNAC30, respectively. Functional characterization via virus-induced gene silencing and overexpression revealed that CiGATA8b could increase disease resistance, and CiGATA12a was able to alleviate oxidative damage. Our results suggest that CiGATA8b and CiGATA12a are associated with stress responses, laying a foundation for understanding the molecular mechanisms of graft healing in pecan.

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