Molecular Identification of Entomopathogenic Nematodes Species and their Endosymbionts in Iraq

An investigation was conducted in two provinces of Iraq to isolate and identify entomopathogenic nematodes (EPNs). Soil samples were collected from agricultural sites across two provinces and tested for nematodes. Two nematodes, Oscheius myriophilus, and Steinernema carpocapse, were collected and molecularly reported. Next-generation sequencing (NGS) technique was used for the first time to identify and report complete mitochondrial genes of the EPNs from Iraq. Both EPNs O. myriophilus and S. carpocapse were recorded in GenBank with accession numbers PQ346375.1 and PQ324366.1respectively. One endosymbiont bacteria Serratia sp., was reported from O. myriophilus. These molecularly identified bacteria used 16S rRNA and 23S rRNA genes and were given accession numbers PQ385864.1 and PQ385865.1, respectively, in GenBank. The results might increase the knowledge of using EPNs as a biological control against insect pests in Iraq.

• Publication year

  2025

• Venue

  IOP Conference Series: Earth and Environment

• Publication date

  2025-04-01

• Fields of study

  Biology, Physics, Environmental Science

• Identifiers
  DOI 10.1088/1755-1315/1487/1/012025
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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