DNA Barcoding as a Tool for Surveying Cytospora Species Associated with Branch Dieback and Canker Diseases of Woody Plants in Canada

Background/Objectives: Branch dieback and canker diseases caused by Cytospora species adversely impact the health of woody plants worldwide. Results: During this survey, 59 Cytospora isolates were obtained from symptomatic trees and shrubs growing in southwest Ontario and Saskatchewan, Canada. A DNA barcoding approach combined with morphological characterization identified 15 known species of Cytospora associated with these diseases: C. chrysosperma, C. curvata, C. euonymina, C. hoffmannii, C. kantschavelii, C. leucosperma, C. leucostoma, C. nitschkeana, C. piceae, C. populina, C. pruinopsis, C. pruinosa, C. ribis, C. schulzeri, and C. sorbina. The most common species isolated from multiple hosts were C. sorbina (10), C. chrysosperma (8), C. nitschkeana (6), and C. pruinosa (6). A wide range of host associations, including non-conifer species, was observed for C. piceae. Conclusions: The obtained results contribute to the study of diversity, host affiliation, geographical distribution, and pathogenicity of Cytospora species occurring on woody plants in both natural habitats and agricultural systems. The findings support the effectiveness of using DNA barcodes in fungal taxonomy and plant pathology studies.

• Publication year

  2025

• Venue

  DNA

• Publication date

  2025-04-21

• Fields of study

  Not labeled

• Identifiers
  DOI 10.3390/dna5020020
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• Morphology and Phylogeny Reveal Three New Species of Cytospora Associated with Tree Cankers in China

  2024cited by this paper
• Diversity of Cytospora Species Associated with Trunk Diseases of Prunus persica (Peach) in Northern China

  2024cited by this paper
• Cytospora: an important genus of canker pathogens.

  2024influential reference
• Identification of Cytospora Species Isolated from Branch Canker Diseases of Woody Plants in Tibet, China

  2024cited by this paper
• First Report of Olive Branch Dieback in Croatia Caused by Cytospora pruinosa Défago

  2023cited by this paper
• The TrEase web service: inferring phylogenetic trees with ease

  2023influential reference
• First Report of Cytospora azerbaijanica Causing Cytospora Canker and Shoot Dieback on Peach (Prunus persica) in California, U.S.A.

  2023cited by this paper
• Re-evaluation of the fungal diversity and pathogenicity of Cytospora species from Populus in China.

  2022cited by this paper
• Multigene phylogeny, morphology, and pathogenicity trials reveal novel Cytospora species involved in perennial canker disease of apple trees in Iran.

  2022cited by this paper
• Fungal Richness of Cytospora Species Associated with Willow Canker Disease in China

  2022cited by this paper
• Assessment of Cytospora Isolates From Conifer Cankers in China, With the Descriptions of Four New Cytospora Species

  2021cited by this paper
• Species Diversity, Mating Strategy and Pathogenicity of Calonectria Species from Diseased Leaves and Soils in the Eucalyptus Plantation in Southern China

  2021cited by this paper
• Olive Twig and Branch Dieback in California Caused by Cytospora oleicola and the Newly Described Species Cytospora olivarum sp. nov.

  2020cited by this paper
• DNA Barcoding of Fungi in the Forest Ecosystem of the Psunj and Papuk Mountains in Croatia

  2020cited by this paper
• High Diversity of Cytospora Associated With Canker and Dieback of Rosaceae in China, With 10 New Species Described

  2020cited by this paper
• Pathogenicity and distribution of two species of Cytospora on Populus tremuloides in portions of the Rocky Mountains and midwest in the United States

  2020cited by this paper
• Cytospora chrysosperma causing branch dieback and canker of black walnut in China

  2020cited by this paper
• ModelTest-NG: A New and Scalable Tool for the Selection of DNA and Protein Evolutionary Models

  2019influential reference
• Cytospora (Diaporthales) in China

  2019cited by this paper
• Cytospora piceae sp. nov. associated with canker disease of Picea crassifolia in China

  2018cited by this paper
• Fungal diversity notes 111–252—taxonomic and phylogenetic contributions to fungal taxa

  2016cited by this paper
• Cytospora from Ulmus pumila in Northern China

  2015cited by this paper
• Cytospora from Salix in northern China.

  2015cited by this paper
• Cytospora species associated with canker disease of three anti-desertification plants in northwestern China

  2015cited by this paper
• Towards a natural classification of Astrosphaeriella-like species; introducing Astrosphaeriellaceae and Pseudoastrosphaeriellaceae fam. nov. and Astrosphaeriellopsis, gen. nov.

  2015cited by this paper
• RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies

  2014influential reference
• MEGA6: Molecular Evolutionary Genetics Analysis version 6.0.

  2013influential reference
• Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi

  2012cited by this paper
• DNA barcoding: a six-question tour to improve users' awareness about the method

  2010cited by this paper
• Cytospora species (Ascomycota, Diaporthales, Valsaceae): introduced and native pathogens of trees in South Africa

  2006cited by this paper
• Phylogenetic relationships and morphology of cytospora species and related teleomorphs ( Ascomycota, Diaporthales, Valsaceae ) from eucalyptus

  2005cited by this paper
• First Report of Cytospora Canker Caused by Cytospora chrysosperma on White Poplar in Israel.

  2004cited by this paper
• Ribosomal DNA sequence divergence and group I introns within the Leucostoma species L. cinctum, L. persoonii, and L. parapersoonii sp. nov., ascomycetes that cause Cytospora canker of fruit trees

  2002cited by this paper
• MRBAYES: Bayesian inference of phylogenetic trees

  2001cited by this paper
• A method for designing primer sets for speciation studies in filamentous ascomycetes

  1999cited by this paper
• CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.

  1994influential reference
• An Empirical Test of Bootstrapping as a Method for Assessing Confidence in Phylogenetic Analysis

  1993cited by this paper
• Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics

  1990cited by this paper
• Integrated approach to controlling Leucostoma canker of peach in Ontario.

  1989cited by this paper

• No citing papers are available for this paper.