Characterization of excitatory synaptic transmission in the retrosplenial cortex of adult mice

The retrosplenial cortex (RSC) plays an important role in navigation, memory and pain. However, there are few studies on excitatory synaptic transmission in the RSC. Here, we used a multi-electrode array recording system (MED64) to study the characteristics of excitatory synaptic transmission in the RSC and the contribution of different types of voltage-gated Ca2+ channels (VGCCs) in excitatory synaptic transmission. We found that glutamate is the major excitatory transmitter for RSC, and postsynaptic alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors contribute to excitatory synaptic transmission. We also found that the N-type calcium channel blocker ω-conotoxin GVIA (ω-Ctx GVIA) had an inhibitory effect on basal synaptic transmission. The inhibitory effect was not consistent across channels, suggesting the actions effect of N-type VGCCs in RSC was inhomogeneous in spatial distribution. Our findings provide strong evidence that excitatory synaptic transmission in the RSC is mainly mediated by AMPA receptors and that N-type VGCCs mediate fast synaptic transmission in the RSC of adult mice.

• Publication year

  2025

• Venue

  Molecular Pain

• Publication date

  2025-05-03

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1177/17448069251335500PMID 40317242PMCID 12171265
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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