Utilisation of stir bar sorptive extraction for the analysis of biogenic amines in wines.

Wine intolerance primarily concerns the body's ability to process histamine, sulphites, tannins, and alcohol. Histamine, a biogenic amine, is involved in immune responses and helps regulate stomach acid. The legal limits for histamine in wine range from 2 to 10 mg/mL. Accurate estimation of histamine requires a sensitive and reliable sample preparation technique. In our study, we applied stir bar sorptive extraction (SBSE) with in situ derivatization using isobutyl chloroformate, followed by gas chromatographic analysis. Under optimal experimental conditions, established through multivariate design of experiments, a limit of detection (LOD) ranging from 5 to 17 μg/L was achieved. The developed SBSE method was used to compare the biogenic amine content in botrytized wines from different regions, including Slovakia, Hungary, France, and Austria. Principal component analysis revealed partial segregation of the samples from France and Hungary.

• Publication year

  2025

• Venue

  Food Chemistry

• Publication date

  2025-05-01

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1016/j.foodchem.2025.144775PMID 40412207
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• A non-target geographical origin screening of botrytized wines through comprehensive two-dimensional gas chromatography coupled with high-resolution mass spectrometry.

  2023cited by this paper
• Current state and future perspectives of supercritical fluid chromatography

  2022cited by this paper
• Optimization of stir bar sorptive extraction (SBSE) and multi-stir bar sorptive extraction (mSBSE) to improve must volatile compounds extraction

  2022cited by this paper
• Ultrahigh-Performance Supercritical Fluid Chromatography and Detection of Multiple Biogenic Amines in Gentamicin Sulfate: Method Development Using Computer-Assisted Modeling.

  2022cited by this paper
• A review of the currently developed analytical methods for the determination of biogenic amines in food products.

  2022cited by this paper
• Enhanced multi-stir bar sorptive extraction for wine analysis: Alteration in headspace mode.

  2022cited by this paper
• Optimization of enantiomer separation in flow-modulated comprehensive two-dimensional gas chromatography by response surface methodology coupled to artificial neural networks: Wine analysis case study.

  2022cited by this paper
• Miniaturized, green salting-out liquid-liquid microextraction coupled with GC-MS used to evaluate biogenic amines in wine samples

  2022cited by this paper
• Green, simple analytical method for biogenic amines determination in fruit juice samples using salting-out assisted liquid-liquid microextraction andgas chromatography-mass spectrometry.

  2022cited by this paper
• Rapid Simultaneous Analysis of Ten Biogenic Amines in Aquatic Products by Ultra-high-performance Supercritical Fluid Chromatography Combined with Mass Spectrometry

  2022cited by this paper
• Slovak Tokaj wines classification with respect to geographical origin by means of one class approaches.

  2021cited by this paper
• Determination of 6 biogenic amines in food using high-performance liquid chromatography-tandem mass spectrometry without derivatization.

  2021cited by this paper
• Liquid Chromatographic Determination of Biogenic Amines in Fish Based on Pyrene Sulfonyl Chloride Pre-Column Derivatization

  2020cited by this paper
• The Importance of Derivatizing Reagent in Chromatography Applications for Biogenic Amine Detection in Food and Beverages

  2020cited by this paper
• Biogenic amines: formation, action and toxicity – a review

  2020cited by this paper
• Dispersive liquid-liquid microextraction combined with gas chromatography–mass spectrometry for in situ determination of biogenic amines in meat: Estimation of meat's freshness

  2019cited by this paper
• Impact of Biogenic Amines on Food Quality and Safety

  2019cited by this paper
• Prediction of the Biogenic Amines Index of Poultry Meat Using an Electronic Nose

  2019cited by this paper
• Literature update of analytical methods for biogenic amines determination in food and beverages

  2018cited by this paper
• The Content of Biogenic Amines in Croatian Wines of Different Geographical Origins

  2018cited by this paper
• Strategies in liquid chromatographic methods for the analysis of biogenic amines without and with derivatization

  2018cited by this paper
• Direct solid phase microextraction combined with gas chromatography - Mass spectrometry for the determination of biogenic amines in wine.

  2018cited by this paper
• Biogenic amines in seafood: a review

  2016cited by this paper
• An in situ derivatization - dispersive liquid-liquid microextraction combined with gas-chromatography - mass spectrometry for determining biogenic amines in home-made fermented alcoholic drinks.

  2016cited by this paper
• Development of a stir bar sorptive extraction method for the determination of volatile compounds in orange juices.

  2016cited by this paper
• Biogenic amines in wine: a review

  2015cited by this paper
• Simultaneous Determination of Seven Biogenic Amines in Foodstuff Samples Using One-Step Fluorescence Labeling and Dispersive Liquid–Liquid Microextraction Followed by HPLC-FLD and Method Optimization Using Response Surface Methodology

  2015cited by this paper
• Chemical derivatization processes applied to amine determination in samples of different matrix composition.

  2015cited by this paper
• Stir bar sorptive extraction coupled to gas chromatography-mass spectrometry for the determination of bisphenols in canned beverages and filling liquids of canned vegetables.

  2012cited by this paper
• A novel dispersive liquid–liquid microextraction (DLLME) gas chromatography-mass spectrometry (GC–MS) method for the determination of eighteen biogenic amines in beer

  2012cited by this paper
• Gas chromatography-mass spectrometry assessment of amines in Port wine and grape juice after fast chloroformate extraction/derivatization.

  2011cited by this paper
• Stir bar sorptive extraction for trace analysis.

  2007cited by this paper
• Optimisation of stir bar sorptive extraction applied to the determination of volatile compounds in vinegars

  2006cited by this paper
• Optimisation of stir bar sorptive extraction applied to the determination of volatile compounds in vinegars.

  2006cited by this paper
• Protection of originality of Tokaji Aszú: amines and organic acids in botrytized wines by high-performance liquid chromatography.

  2005cited by this paper
• Study of biologically active amines in grapes and wines by HPLC

  2000cited by this paper
• Occurrence and formation of biologically active amines in foods.

  1990cited by this paper
• Determination of piperazine in working atmosphere and in human urine using derivatization and capillary gas chromatography with nitrogen- and mass-selective detection.

  1986cited by this paper

• Biogenic amines in fermented foods: A comprehensive review from formation pathways, risk analysis, detection technologies to control measures.

  2026cites this paper
• Profiling and risk assessment of biogenic amines in Chinese wines: Content variations driven by multifactorial influences.

  2025cites this paper