Melatonin Regulates Clusterin to Reconstruct the Blood-Testis Barrier Disrupted by Atrazine.

Atrazine (ATZ), a commonly used triazine herbicide, is known to impair male reproductive function. Melatonin (MLT) is an endogenous hormone synthesized by the pineal gland. Its lipophilic nature enables it to freely penetrate the blood-testis barrier (BTB) and exert direct effects. However, it remains unclear whether MLT can prevent ATZ-induced impairment of BTB function. Pubertal male mice were randomly assigned to four groups and treated via gavage for 21 days: control group (Con), 5 mg/kg melatonin group (MLT), 170 mg/kg atrazine group (ATZ), and cotreatment group (ATZ+MLT). Furthermore, BTB-associated proteins, transepithelial electrical resistance, and cell adhesion assays were performed in TM4 cells treated with siClusterin, pcDNA3.1-Clusterin, and AKT inhibitor VIII to evaluate BTB function in vitro. Our findings demonstrate that MLT effectively restores ATZ-induced reductions in BTB protein levels and function in both in vivo and in vitro models. Moreover, MLT mitigated ATZ-induced BTB damage by reactivating the Clusterin/AKT signaling pathway.

• Publication year

  2025

• Venue

  Journal of Agricultural and Food Chemistry

• Publication date

  2025-05-23

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1021/acs.jafc.5c00599PMID 40407246
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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