HLA‐Typing of Donor‐Origin Cells Enriched From Urine Cell Culture of Kidney Transplanted Recipients

The incomplete or lack of histocompatibility information constitutes a barrier for the early detection and management of de novo donor‐specific antibodies (DSA). To improve the quantity and quality of DNA materials for HLA typing, we developed a non‐invasive culture‐based method, using DNA extracted from enriched donor‐derived kidney stem cells (DKSC) selectively cultured from the urine of kidney transplant receipients (KTR) to allow high‐resolution typing by next‐generation sequencing. This prospective proof‐of‐concept study evaluated the feasibility and performance of this approach. DKSC were enriched from the urine of 60 KTRs. DNA extracted from culture‐enriched DKSC showed significantly higher concentration and better quality than unbound cells, and with identical short tandem repeat (STR) and 100% concordance compared to that obtained from peripheral blood. Our results suggest that cultured‐enriched DKSC are non‐invasive and useful for determining HLA and other genes for KTRs where donor information is limited or lacking.

• Publication year

  2025

• Venue

  HLA

• Publication date

  2025-05-29

• Fields of study

  Medicine

• Identifiers
  DOI 10.1111/tan.70253PMID 40439142
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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