Modulating lipid metabolism for enhancement of coenzyme Q10 production in Rhodobacter sphaeroides.

Coenzyme Q10 (CoQ10), a lipid-soluble isoprenylated benzoquinone essential for electron transfer, is synthesized in the inner mitochondrial membrane of eukaryotes and plasma membrane of prokaryotes like Rhodobacter sphaeroides. Despite its natural production capacity, industrial-scale CoQ10 synthesis remains limited by ineffective metabolic regulation and scarce genetic tools in R. sphaeroides. This study investigated the relationship between CoQ10 and intracellular lipid accumulation to enhance production. The engineered strains with higher cardiolipin (CL) levels showed enlarged membrane surfaces, synergistically increasing CoQ10 production. However, increased triacylglycerol (TAG) accumulation reduced CoQ10 yields. Disrupting phospholipase D (PLD) and lysophospholipase (PLDB) genes, which reduce phospholipid hydrolysis, further improved CoQ10 biosynthesis. The optimized strain RS-SQCL04, incorporating all the effective strategies, achieved 824 mg/L CoQ10 via fed-batch fermentation, a 57.9 % increase over the control. This study provides a promising strategy for industrial CoQ10 production through phospholipid engineering in R. sphaeroides.

• Publication year

  2025

• Venue

  Bioresource Technology

• Publication date

  2025-06-09

• Fields of study

  Biology, Chemistry, Engineering, Environmental Science, Medicine

• Identifiers
  DOI 10.1016/j.biortech.2025.132794PMID 40499666
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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