The establishment of organoids and their applications

Organoids are three-dimensional (3D) cell culture models that replicate key structural and functional features of the organ they represent. Recently, research on the development and application of organoids has grown substantially. This review employs a literature review approach to summarize advances in organoids establishment and their applications, with a particular focus on traditional Chinese medicine (TCM) research. A PubMed search yielded 750 articles, of which 100 were analyzed, indicating that organoids can be derived from both healthy and cancerous tissues. They are primarily utilized in cancer research for studying disease mechanisms, drug screening, personalized treatment, and the development of biobanks. Additionally, organoids have emerging applications in TCM research. In summary, organoids hold significant promise across various domains, particularly in cancer research.

• Publication year

  2025

• Venue

  New Discovery

• Publication date

  2025-06-30

• Fields of study

  Not labeled

• Identifiers
  DOI 10.61958/ndnu9408
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• Research progress and application of liver organoids for disease modeling and regenerative therapy

  2024cited by this paper
• Patient tissue-derived FGFR4-variant and wild-type colorectal cancer organoid development and anticancer drug sensitivity testing

  2024cited by this paper
• Astragalus polysaccharide promotes the regeneration of intestinal stem cells through HIF‐1 signalling pathway

  2023cited by this paper
• Applications of lung cancer organoids in precision medicine: from bench to bedside

  2023cited by this paper
• Codonopsis pilosula polysaccharide alleviates rotenone-induced murine brain organoids death through downregulation of gene body DNA methylation modification in the ZIC4/PGM5/CAMTA1 axis

  2023cited by this paper
• Models for evaluating glioblastoma invasion along white matter tracts.

  2023cited by this paper
• Pharmaco-proteogenomic characterization of liver cancer organoids for precision oncology

  2023cited by this paper
• Paeoniflorin promotes intestinal stem cell-mediated epithelial regeneration and repair via PI3K-AKT-mTOR signalling in ulcerative colitis.

  2023cited by this paper
• Cancer organoid co-culture model system: Novel approach to guide precision medicine

  2023cited by this paper
• Tumor organoid biobank-new platform for medical research

  2023cited by this paper
• Liver organoids and their application in liver cancer research

  2023cited by this paper
• Extraction, structure, and activity of polysaccharide from Radix astragali.

  2022cited by this paper
• Self-Assembled Matrigel-Free iPSC-Derived Liver Organoids Demonstrate Wide-Ranging Highly Differentiated Liver Functions

  2022cited by this paper
• Hepatic Organoid-Based High-Content Imaging Boosts Evaluation of Stereoisomerism-Dependent Hepatotoxicity of Stilbenes in Herbal Medicines

  2022cited by this paper
• Systematic evaluation of colorectal cancer organoid system by single-cell RNA-Seq analysis

  2022cited by this paper
• Organoid screening reveals epigenetic vulnerabilities in human colorectal cancer

  2022cited by this paper
• Diagnosis and Treatment of Metastatic Colorectal Cancer: A Review.

  2021cited by this paper
• Organoids from patient biopsy samples can predict the response of BC patients to neoadjuvant chemotherapy

  2021cited by this paper
• Application of CRISPR-Cas9 based gene editing to study the pathogenesis of colon and liver cancer using organoids

  2021cited by this paper
• Application of Organoid Models in Prostate Cancer Research

  2021cited by this paper
• Drug resistance in colorectal cancer: An epigenetic overview.

  2021cited by this paper
• A Novel Orthotopic Liver Cancer Model for Creating a Human-like Tumor Microenvironment

  2021cited by this paper
• Publisher Correction: Human heart-forming organoids recapitulate early heart and foregut development

  2021cited by this paper
• Long-term culture, genetic manipulation and xenotransplantation of human normal and breast cancer organoids

  2021cited by this paper
• Cancer Statistics, 2021

  2021cited by this paper
• TIP5 primes prostate luminal cells for the oncogenic transformation mediated by PTEN-loss

  2020cited by this paper
• Targeting Acid Ceramidase to Improve the Radiosensitivity of Rectal Cancer

  2020cited by this paper
• PDX-derived organoids model in vivo drug response and secrete biomarkers

  2020cited by this paper
• Establishment of patient-derived cancer organoids for drug-screening applications

  2020cited by this paper
• Outgrowth of erlotinib-resistant subpopulations recapitulated in patient-derived lung tumor spheroids and organoids

  2020cited by this paper
• Are Organoids Ready for Prime Time?

  2020cited by this paper
• Codonopsis pilosula polysaccharide attenuates Aβ toxicity and cognitive defects in APP/PS1 mice

  2020cited by this paper
• Human organoids: model systems for human biology and medicine

  2020cited by this paper
• Patient-derived tumor organoid predicts drugs response in glioblastoma: A step forward in personalized cancer therapy?

  2020cited by this paper
• Hedgehog signaling promotes sorafenib resistance in hepatocellular carcinoma patient-derived organoids

  2020cited by this paper
• A rectal cancer organoid platform to study individual responses to chemoradiation

  2019cited by this paper
• Generation of hepatobiliary organoids from human induced pluripotent stem cells.

  2019cited by this paper
• Establishment and Morphological Characterization of Patient-Derived Organoids from Breast Cancer

  2019cited by this paper
• Patient-derived lung cancer organoids as in vitro cancer models for therapeutic screening

  2019cited by this paper
• Breast cancer statistics, 2019

  2019cited by this paper
• Modeling breast cancer using CRISPR/Cas9-mediated engineering of human breast organoids.

  2019cited by this paper
• Patient-derived organoids can predict response to chemotherapy in metastatic colorectal cancer patients

  2019cited by this paper
• Organoid technology and applications in cancer research

  2018cited by this paper
• APOE4 Causes Widespread Molecular and Cellular Alterations Associated with Alzheimer's Disease Phenotypes in Human iPSC-Derived Brain Cell Types.

  2018cited by this paper
• A Living Biobank of Breast Cancer Organoids Captures Disease Heterogeneity.

  2018cited by this paper
• Organoid technology in disease modelling, drug development, personalized treatment and regeneration medicine

  2018cited by this paper
• Progress and potential in organoid research

  2018cited by this paper
• Drug resistance and new therapies in colorectal cancer

  2018cited by this paper
• Patient-derived organoids model treatment response of metastatic gastrointestinal cancers

  2018cited by this paper
• The rise of three-dimensional human brain cultures

  2018cited by this paper
• Erratum: Mapping the global influence of published research on industry and innovation

  2018cited by this paper
• Personalized Cancer Medicine: An Organoid Approach.

  2018cited by this paper
• A Cellular Anatomy of the Normal Adult Human Prostate and Prostatic Urethra

  2018cited by this paper
• Building Models of Brain Disorders with Three-Dimensional Organoids.

  2018cited by this paper
• Clonal Heterogeneity and Tumor Evolution: Past, Present, and the Future.

  2017cited by this paper
• Interrogating open issues in cancer precision medicine with patient-derived xenografts

  2017cited by this paper
• iPSC-Derived Human Microglia-like Cells to Study Neurological Diseases.

  2017cited by this paper
• Mechanisms of Drug-Induced Hepatotoxicity.

  2017cited by this paper
• The PIAS3-Smurf2 sumoylation pathway suppresses breast cancer organoid invasiveness

  2017cited by this paper
• Next generation organoids for biomedical research and applications.

  2017cited by this paper
• Cell diversity and network dynamics in photosensitive human brain organoids

  2017cited by this paper
• Human Astrocyte Maturation Captured in 3D Cerebral Cortical Spheroids Derived from Pluripotent Stem Cells.

  2017cited by this paper
• Modeling Development and Disease with Organoids.

  2016cited by this paper
• Novel human hepatic organoid model enables testing of drug-induced liver fibrosis in vitro.

  2016cited by this paper
• Regulation of embryonic neurogenesis by germinal zone vasculature

  2016cited by this paper
• Self-organization of polarized cerebellar tissue in 3D culture of human pluripotent stem cells.

  2015cited by this paper
• Prospective derivation of a living organoid biobank of colorectal cancer patients.

  2015cited by this paper
• Human cerebral organoids recapitulate gene expression programs of fetal neocortex development

  2015cited by this paper
• Modeling mouse and human development using organoid cultures

  2015cited by this paper
• Cerebral organoids model human brain development and microcephaly

  2014cited by this paper
• Drug-Induced Hepatotoxicity: Metabolic, Genetic and Immunological Basis

  2014cited by this paper
• Organogenesis in a dish: Modeling development and disease using organoid technologies

  2014cited by this paper
• Single luminal epithelial progenitors can generate prostate organoids in culture

  2014cited by this paper
• Modeling human development and disease in pluripotent stem cell-derived gastric organoids

  2014cited by this paper
• Human pluripotent stem cell culture: considerations for maintenance, expansion, and therapeutics.

  2014cited by this paper
• Hematogenous dissemination of glioblastoma multiforme

  2014cited by this paper
• In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration

  2013cited by this paper
• Invasion as target for therapy of glioblastoma multiforme.

  2013cited by this paper
• Growing Self-Organizing Mini-Guts from a Single Intestinal Stem Cell: Mechanism and Applications

  2013cited by this paper
• Slug and Sox9 cooperatively determine the mammary stem cell state.

  2012cited by this paper
• Promotion of the efficient metabolic maturation of human pluripotent stem cell-derived hepatocytes by correcting specification defects

  2012cited by this paper
• Rapid generation of mature hepatocyte‐like cells from human induced pluripotent stem cells by an efficient three‐step protocol

  2012cited by this paper
• Functional engraftment of colon epithelium expanded in vitro from a single adult Lgr5+ stem cell

  2012cited by this paper
• Self-formation of optic cups and storable stratified neural retina from human ESCs.

  2012cited by this paper
• Neurovascular pathways to neurodegeneration in Alzheimer's disease and other disorders

  2011cited by this paper
• Chemical Constituents and Bioactivities of Plants from the Genus Paeonia

  2010cited by this paper
• Brain angiogenesis in developmental and pathological processes: regulation, molecular and cellular communication at the neurovascular interface

  2009cited by this paper
• Single Lgr5 stem cells build crypt–villus structures in vitro without a mesenchymal niche

  2009cited by this paper
• Efficient generation of hepatocyte-like cells from human induced pluripotent stem cells

  2009cited by this paper
• Article Endothelial Nt-3 Delivered by Vasculature and Csf Promotes Quiescence of Subependymal Neural Stem Cells through Nitric Oxide Induction

  year unknowncited by this paper

• No citing papers are available for this paper.