Expression, purification and characterization of recombinant mitogen-activated protein kinase kinases.

No abstract is available for this paper.

• Publication year

  1994

• Venue

  Biochemical Journal

• Publication date

  1994-10-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1042/BJ3030105PMID 7945229PMCID PMC1137563
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Mitogen-activated protein kinase kinase 1 (MKK1) is negatively regulated by threonine phosphorylation

  1994cited by this paper
• Activation of MEK family kinases requires phosphorylation of two conserved Ser/Thr residues.

  1994cited by this paper
• Normal and oncogenic p21ras proteins bind to the amino-terminal regulatory domain of c-Raf-1

  1993cited by this paper
• Complexes of Ras.GTP with Raf-1 and mitogen-activated protein kinase kinase.

  1993cited by this paper
• The mitogen-activated protein kinase signal transduction pathway.

  1993cited by this paper
• Molecular structure of a protein-tyrosine/threonine kinase activating p42 mitogen-activated protein (MAP) kinase: MAP kinase kinase.

  1993cited by this paper
• Raf-1 forms a stable complex with Mek1 and activates Mek1 by serine phosphorylation.

  1993cited by this paper
• The interaction of SV40 small tumor antigen with protein phosphatase 2A stimulates the map kinase pathway and induces cell proliferation.

  1993cited by this paper
• Signal transduction via the MAP kinases: proceed at your own RSK.

  1993cited by this paper
• Active baculovirus recombinant p70s6k and p85s6k produced as a function of the infectious response.

  1993cited by this paper
• Identification of insulin-stimulated protein kinase-1 as the rabbit equivalent of rskmo-2. Identification of two threonines phosphorylated during activation by mitogen-activated protein kinase.

  1993cited by this paper
• Growth factors induce nuclear translocation of MAP kinases (p42mapk and p44mapk) but not of their activator MAP kinase kinase (p45mapkk) in fibroblasts

  1993cited by this paper
• A divergence in the MAP kinase regulatory network defined by MEK kinase and Raf

  1993cited by this paper
• Identification and characterization of a new mammalian mitogen-activated protein kinase kinase, MKK2

  1993cited by this paper
• Cloning and characterization of two distinct human extracellular signal-regulated kinase activator kinases, MEK1 and MEK2.

  1993cited by this paper
• Serum-induced translocation of mitogen-activated protein kinase to the cell surface ruffling membrane and the nucleus

  1993cited by this paper
• Mitogen-activated protein kinases p42mapk and p44mapk are required for fibroblast proliferation.

  1993cited by this paper
• Critical tyrosine residues regulate the enzymatic and biological activity of Raf-1 kinase

  1993cited by this paper
• Ordered phosphorylation of p42mapk by MAP kinase kinase

  1992cited by this paper
• Phosphorylation of transcription factor p62TCF by MAP kinase stimulates ternary complex formation at c-fos promoter

  1992cited by this paper
• Reconstitution of interactions between the Src tyrosine kinases and Ras GTPase-activating protein using a baculovirus expression system.

  1992cited by this paper
• Signal transduction within the nucleus by mitogen-activated protein kinase.

  1992cited by this paper
• Nuclear localization and regulation of erk- and rsk-encoded protein kinases

  1992cited by this paper
• Sustained activation of the mitogen-activated protein (MAP) kinase cascade may be required for differentiation of PC12 cells. Comparison of the effects of nerve growth factor and epidermal growth factor.

  1992cited by this paper
• Both p21ras and pp60v-src are required, but neither alone is sufficient, to activate the Raf-1 kinase.

  1992cited by this paper
• Identification of the regulatory phosphorylation sites in pp42/mitogen‐activated protein kinase (MAP kinase).

  1991cited by this paper
• ERKs, extracellular signal-regulated MAP-2 kinases.

  1991cited by this paper
• Definition of the human raf amino-terminal regulatory region by deletion mutagenesis

  1989cited by this paper
• Nucleotide sequence of the yeast regulatory gene STE7 predicts a protein homologous to protein kinases.

  1986cited by this paper
• Affinity labeling of cAMP-dependent protein kinase with p-fluorosulfonylbenzoyl adenosine. Covalent modification of lysine 71.

  1981cited by this paper

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• Co-expression vs. co-infection using baculovirus expression vectors in insect cell culture: Benefits and drawbacks

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• Deciphering Protein Kinase Specificity through Large-scale Analysis of Materials Supplemental Deciphering Protein Kinase Specificity through Large-scale Analysis of Yeast Phosphorylation Site Motifs

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• Approaches for monitoring signal transduction changes in normal and cancer cells.

  2007cites this paper
• Expression and purification of phosphorylated and non-phosphorylated human MEK1.

  2007cites this paper
• Follicle-stimulating Hormone Activates Extracellular Signal-regulated Kinase but Not Extracellular Signal-regulated Kinase Kinase through a 100-kDa Phosphotyrosine Phosphatase*

  2003cites this paper
• Effects of inducible MEK1 activation on the cytokine dependency of lymphoid cells

  2001cites this paper
• Kinase Suppressor of Ras Signals through Thr269 of c-Raf-1*

  2001cites this paper
• A conditionally-active form of MEK1 results in autocrine transformation of human and mouse hematopoietic cells

  2000cites this paper
• Synergy between Raf and BCL2 in abrogating the cytokine dependency of hematopoietic cells

  2000cites this paper
• Distinct, constitutively active MAPK phosphatases function in Xenopus oocytes: implications for p42 MAPK regulation In vivo.

  1999influential citation
• Signal transduction through MAP kinase cascades.

  1998cites this paper
• Microtubule-interfering Agents Activate c-Jun N-terminal Kinase/Stress-activated Protein Kinase through Both Ras and Apoptosis Signal-regulating Kinase Pathways*

  1998cites this paper
• The immunophilin FKBP65 forms an association with the serine/threonine kinase c-Raf-1.

  1998cites this paper
• Prostaglandin F2alpha stimulates the Raf/MEK1/mitogen-activated protein kinase signaling cascade in bovine luteal cells.

  1998cites this paper
• Prostaglandin F2α Stimulates the Raf/MEK1/Mitogen-Activated Protein Kinase Signaling Cascade in Bovine Luteal Cells.

  1998cites this paper
• Identification of Raf‐1 Ser621 kinase activity from NIH 3T3 cells as AMP‐activated protein kinase

  1997cites this paper
• Mammalian Raf‐1 is activated by mutations that restore Raf signaling in Drosophila

  1997cites this paper
• The hsp90-binding Antibiotic Geldanamycin Decreases Raf Levels and Epidermal Growth Factor Signaling without Disrupting Formation of Signaling Complexes or Reducing the Specific Enzymatic Activity of Raf Kinase*

  1997cites this paper
• Interdependent domains controlling the enzymatic activity of mitogen-activated protein kinase kinase 1.

  1996cites this paper
• KSR modulates signal propagation within the MAPK cascade.

  1996cites this paper
• Activation of Raf by ionizing radiation

  1996cites this paper
• A proline-rich sequence unique to MEK1 and MEK2 is required for raf binding and regulates MEK function

  1995cites this paper
• mechanisms in vitro . Ras-dependent and Ras-independent Regulation of Raf-1 and Raf-1 mutants

  1995cites this paper
• Phosphorylation of Tristetraprolin, a Potential Zinc Finger Transcription Factor, by Mitogen Stimulation in Intact Cells and by Mitogen-activated Protein Kinase in Vitro(*)

  1995cites this paper
• Raf-1 N-terminal sequences necessary for Ras-Raf interaction and signal transduction

  1995cites this paper
• Regulation of Raf-1 and Raf-1 mutants by Ras-dependent and Ras-independent mechanisms in vitro

  1995cites this paper
• Activation of Raf-1 by Ras in intact cells.

  1995cites this paper
• 14-3-3 is not essential for Raf-1 function: identification of Raf-1 proteins that are biologically activated in a 14-3-3- and Ras-independent manner

  1995cites this paper
• Reversal of Raf-1 activation by purified and membrane-associated protein phosphatases.

  1995cites this paper
• Functional Mapping of the N-terminal Regulatory Domain in the Human Raf-1 Protein Kinase (*)

  1995cites this paper
• The MPM-2 antibody inhibits mitogen-activated protein kinase activity by binding to an epitope containing phosphothreonine-183.

  1994cites this paper