Integrating Full-Length and Second-Generation Transcriptomes to Elucidate the ApNPV-Induced Transcriptional Reprogramming in Antheraea pernyi Midgut

Simple Summary The midgut of Antheraea pernyi serves as a primary defense barrier against A. pernyi nuclear polyhedrosis virus (ApNPV); however, the molecular interplay between the virus and host defense remains incompletely characterized. In this investigation, PacBio Iso-Seq and RNA-seq data were integrated for comprehensive profiling of the midgut transcriptome and dynamic responses to orally infected viruses that had formed polyhedra in the hemolymph. Many metabolism-related genes were downregulated. However, genes related to A. pernyi cytoplasmic translation, autophagy, and apoptosis were found to be upregulated. In addition, several differentially expressed long non-coding RNAs and transcription factors were identified. These results provide initial insights into how ApNPV affects host resources to facilitate viral replication and midgut escape.

• Publication year

  2025

• Venue

  Insects

• Publication date

  2025-07-31

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.3390/insects16080792PMID 40870592PMCID 12386421
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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