Assessment of Fusarium avenaceum inoculation methods for consistent development of pea root rot under greenhouse conditions

Abstract Fusarium avenaceum (Fave) is the most dominant pathogen of the pea root rot complex in the Canadian prairies. Its increasing prevalence and broad host range present significant challenges, underscoring the urgent need for research to develop effective management strategies including breeding for resistance. A critical aspect of efficient pathogen studies and screening pea lines for resistance under controlled conditions is selecting an appropriate inoculation method. In previous experiments, we observed that classical Fave inoculation methods such as seed inoculation either lacked uniform infection or killed plants before emergence. Therefore, we tested different Fave conidia inoculation methods to assess disease development in various pea lines after Fave exposure and refined these methods to develop an optimized indoor screening protocol for Fave. Among the tested methods, soil inoculation with Fave conidia resulted in the most consistent and uniform disease symptoms across repeated experiments. This method could be most effective for germplasm screening for partial resistance. A modified seed inoculation method also resulted in moderate disease levels, making it the most effective for testing the virulence of different isolates of Fave on the same genotype. Root soaking resulted in uniform but low disease severity and can be used for studying host–pathogen interactions where observation during the infection process is important.

• Publication year

  2025

• Venue

  Canadian journal of plant pathology

• Publication date

  2025-08-13

• Fields of study

  Not labeled

• Identifiers
  DOI 10.1080/07060661.2025.2537831
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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