Nobiletin Modulates Adipocyte-Derived Exosomal miRNA to Improve Liver Lipid Metabolism in Obese Mice

This study examines the antiobesity properties of nobiletin through its influence on extracellular vesicles, particularly exosomes. Nob-ELVs (nobiletin with adipocyte-derived extracellular vesicles) significantly reduce fat accumulation in adipocytes without compromising cell viability. In vitro studies showed that introducing Nob-ELVs to 3T3-L1 preadipocytes led to marked reductions in fat accumulation. RNA sequencing indicated that Nobiletin alters the exosomal miRNA profile, downregulating miR-802-5p and others while upregulating miR-574-5p and related miRNAs. In a high-fat diet mouse model, Nob-ELVs prevented significant weight gain and improved food conversion efficiency. Mice receiving Nob-ELVs exhibited reduced hepatic lipid droplet accumulation and altered protein expressions, enhancing SIRT1 and FGF21 while suppressing ACC, FASN, and CD36. These findings suggest that Nob-ELVs inhibit fat accumulation and enhance lipid metabolism by modulating exosomal miRNAs, offering insights for new obesity treatment strategies.

• Publication year

  2025

• Venue

  Journal of Agricultural and Food Chemistry

• Publication date

  2025-08-19

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1021/acs.jafc.5c06392PMID 40831213PMCID 12412145
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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