Characterization of MADS-box genes in Chinese cherry and overexpression of CpMADS2 promoting early flowering in transgenic Arabidopsis.

The Chinese cherry (Cerasus pseudocerasus Lindl.) cv. 'Manaohong', a distinctive cultivar indigenous to Guizhou Province, China, possesses significant nutritional and economic value. In our previous studies, we observed an abnormal flowering phenomenon in the 'Manaohong' cherry. The MADS-box gene family plays a pivotal role in regulating flowering time and the floral organs development in plants. In this study, we identified 51 CpMADS genes. Transcriptome analysis revealed that the PI-like gene CpMADS2 was significantly differentially expressed between normal flowers (NF) and abnormal flowers (AF) in Chinese cherry. To further investigate the functional role of CpMADS2, it was cloned from the Chinese cherry. Overexpression of CpMADS2 in Arabidopsis thaliana exhibited earlier flowering compared to wild-type. Yeast two-hybrid (Y2H) assay and luciferase complementation assay (LCA) confirmed that CpMADS2 interacts with the CpFT, suggesting that CpMADS2 may promotes flowering by interacting with CpFT. This study provides a theoretical foundation for further research on the role of CpMADS2 in regulating abnormal flowering in Chinese cherry.

• Publication year

  2025

• Venue

  Journal of plant physiology

• Publication date

  2025-09-01

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1016/j.jplph.2025.154583PMID 40840125
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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