Purification and analysis of "monofocal" antibody.

“Monofocal” antibody against the 2,4-dinitrophenyl (DNP) haptenic group was obtained from the culture fluids of splenic fragments stimulated in vitro. The antibody was purified and compared with purified serum anti-DNP antibody. The “monofocal” antibodies were shown to bind hapten homogeneously and to have a more restricted electrophoretic mobility than serum antibody. These “monofocal” antibodies differed markedly from one another in their electrophoretic mobilities, affinity for hapten and ease of purification from an immunoadsorbent. From these results it was concluded that serum antibody represents a pool of the products of a variety of clones each producing a homogeneous antibody population.

• Publication year

  1971

• Venue

  Journal of Immunology

• Publication date

  1971-05-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.4049/jimmunol.106.5.1345PMID 5103351
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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