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Genotoxicity of the Neonicotinoid Insecticide Poncho (Clothianidin) on CD1 Mice Based on Alkaline Comet and Micronucleus Assays

M. Calderón-Segura,J. A. M. Rojas,M. Brito,Manuel TecCab,María del CarmenCalderón-Ezquerro,S. Gómez-Arroyo

Published 2015 in Unknown venue

ABSTRACT

Poncho is a commercial formulation of neonicotinoid insecticides and a new agro‐ chemical in Mexico, and it has the active ingredient clothianidin. The genotoxic effects of this commercial formulation of clothianidin on CD1 male mice were analyzed using micronucleus and comet alkaline assays. Three concentrations of clothianidin (20, 40, and 80 mg/kg/body weight) as well as negative (deionized water) and positive controls (cyclophosphamide 40 mg/g/body weight) were intraperitoneally injected into groups of mice every 3 days for 21 days. Peripheral blood samples were drawn from the caudal vein and divided to carry out the comet alkaline and micronuclei assays. DNA damage was evaluated using three genotoxicity parameters: the comet frequency, the tail length, and the moment from 100 nuclei. Additionally, the micronuclei frequency was quantified in 2000 peripheral blood erythrocytes using Giemsa stain. The results of the comet assay showed that the neonicotinoid insecticide Poncho leads to a significant increase in these three genotoxic parameters and in micronuclei frequency in the peripheral blood erythrocytes of mice treated with either concentration as compared with negative controls. At 80 mg/kg/body weight of Poncho, higher micronuclei frequencies and many more DNA strand breaks were observed compared with the negative controls. This study demonstrates that the commercial neonicotinoid insecticide Poncho induces genotoxic effects in CD1 male mice. © 2015 The Author(s). Licensee InTech. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PUBLICATION RECORD

  • Publication year

    2015

  • Venue

    Unknown venue

  • Publication date

    2015-07-22

  • Fields of study

    Biology, Medicine, Chemistry, Environmental Science

  • Identifiers
    DOI 10.5772/61174
  • External record

    Open on Semantic Scholar

  • Source metadata

    Semantic Scholar

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