GluK2 kainate receptor subunit-selective, potentiating RNA aptamer

Kainate receptors are a subtype of ionotropic glutamate receptors. Potent and selective modulatory agents of kainate receptors are potential drug candidates for treatment of various neurological diseases involving receptor dysregulation. Here, we report an RNA aptamer that selectively potentiates GluK2, a key kainate receptor subunit. The potentiating aptamer, termed U9, is designed by changing the sequence of the predecessor RNA, which is an inhibitor without subunit selectivity, leading to the change of function: U9 selectively potentiates GluK2 with an EC50 of ~ 210 nM. Our study demonstrates a potential utility of combining molecular docking with functional assay for a template-based discovery of potent, single-subunit selective RNA aptamers.

• Publication year

  2025

• Venue

  Scientific Reports

• Publication date

  2025-09-11

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1038/s41598-025-15323-yPMID 40935837PMCID 12426214
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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