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Assessing Hospital Cleaning Effectiveness Using Fluorescence: A Proof-of-Concept Study and Comparison to ATP Testing.

Sarah Fieldhouse,Benhur Bakhtiari Bastaki,A. Ledgerton,P. Clarke,Trystan Lewis

Published 2025 in Journal of Hospital Infection

ABSTRACT

Visual inspections are used to assess hospital cleanliness as visible contamination may present health risks and influence perceptions of care quality. Problematically, many contaminants are invisible to the naked eye, limiting the reliability of visual checks. Many invisible substances, however, fluoresce - emit visible light after absorbing electromagnetic radiation. Portable torches can detect fluorescent substances 'in situ', offering a potential method to enhance cleaning practices. This study has evaluated fluorescence as a tool for identifying general invisible contamination after hospital cleaning. Visibly clean surfaces in seven single-occupancy patient rooms and two six-bed wards across two NHS hospitals were examined using a portable high-intensity blue and UV light torch. Adenosine triphosphate levels in fluorescent and non-fluorescent areas were taken as a recognised cleaning monitoring tool and statistically analysed using Wilcoxon Signed-Rank tests. Fluorescent contamination that was invisible to the naked eye was found on every surface. ATP Relative Light Unit (RLU) levels were statistically different (higher) in fluorescent substances compared to non-fluorescent (p ≤ 0.05) with large effect sizes on patient chairs, bed frames, overbed tables, bedside units, and pillows, but not toilets, sinks, or commodes. The mean RLU measurement was 161 in fluorescent areas compared to 33 RLU in the control areas. Fluorescence detected alternative contamination which could present toxic risk to humans, possibly cleaning fluid and/or drug contaminated residue, which frequently contain fluorescent constituents. This was an important finding since relying solely on ATP detection may overlook significant contamination risks. Further work to evaluate the method as a cleaning aid is encouraged.

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